Ectomycorrhizal inoculum and inoculation techniques

I Repáč - Diversity and biotechnology of ectomycorrhizae, 2011 - Springer
… For production of alginate-bead inoculum, fungul cultures are grown in liquid medium.
The mycelial pellets are washed in tap water, homogenized in a blender for 5–10 s, and …

Development of growth media for solid substrate propagation of ectomycorrhizal fungi for inoculation of Norway spruce (Picea abies) seedlings

I Vuorinen, L Hamberg, M Müller, P Seiskari… - Mycorrhiza, 2015 - Springer
… of ectomycorrhizal (ECM) fungal inoculum by solid state fermentation. Development of the
medium was … Forty ml of homogenized liquid culture was used per kg of nutrient medium. The …

Some factors influencing viability of ectomycorrhizal fungal inoculum

FF Lapeyrie, G Bruchet - New phytologist, 1985 - Wiley Online Library
inoculum in liquid culture media, but the transition from culture vessels to semi-natural plant
growth media is … Factors which affect the quality of the inoculum may include the age of the …

Effects of Different Ectomycorrhizal Fungal Inoculates on the Growth of Pinus tabulaeformis Seedlings under Greenhouse Conditions

N Lu, M Yu, M Cui, Z Luo, Y Feng, S Cao, Y Sun, Y Li - Forests, 2016 - mdpi.com
… ) saturating inoculate in an MMN liquid media in … inoculated treatments were applied for a
total volume of 600 mL—a mixture of mycorrhizal inoculum and sterilized mycorrhizal inoculum

Inoculant production of ectomycorrhizal fungi by solid and submerged fermentations

MJ Rossi, A Furigo Jr, VL Oliveira - Food Technology and …, 2007 - hrcak.srce.hr
… inoculants is the lack of information on the biochemistry and physiology of ectomycorrhizal
fungi while growing in liquid medium and on the engineering process in bioreactors. In the …

Inoculum production of the ectomycorrhizal fungus Pisolithus microcarpus in an airlift bioreactor

M Rossi, J Souza, V Oliveira - Applied microbiology and biotechnology, 2002 - Springer
inoculum production method of the ectomycorrhizal fungus Pisolithus microcarpus (isolate
UFSC-Pt116) using liquid … Before inoculation, bioreactor and culture medium were sterilized …

Inoculation of containerized Pinus pinea L. seedlings with seven ectomycorrhizal fungi

A Rincón, IF Alvarez, J Pera - Mycorrhiza, 2001 - Springer
… Pisolithus tinctorius applied as a spore inoculum formed ectomycorrhizas at a frequency of …
20 min, 120C) moistened with 350 ml of liquid MMN medium (glucose reduced to 2.5 g/l). The …

Sustained in vitro root development obtained in Pinus pinea L. inoculated with ectomycorrhizal fungi

P Oliveira, J Barriga, C Cavaleiro, A Peixe, AZ Potes - Forestry, 2003 - academic.oup.com
… of some inoculated plants had ectomycorrhizas from the … culturing rooted microshoots
with ectomycorrhizal fungi can be … each stock culture was transferred to liquid WPM medium

Mycorrhizal synthesis of four ectomycorrhizal fungi in potted Populus maximowiczii seedlings

K Obase, Y Tamai, T Yajima, T Miyamoto - Mycoscience, 2009 - jstage.jst.go.jp
liquid medium containing cultured ECM fungal mycelia. Control seedlings were soaked in
modified MMN liquid medium … between these ECM fungi as inoculum and P. maximowiczii …

Maintenance and preservation of ectomycorrhizal and arbuscular mycorrhizal fungi

I Lalaymia, S Cranenbrouck, S Declerck - Mycorrhiza, 2014 - Springer
… be used (direct immersion in liquid nitrogen or freezing at … and inoculated into a 2-ml sterile
polypropylene cryovial containing 750 μl of sterilized (121 C for 15 min) MFM agar medium