… editing characteristics. Here, we describe a protocol for using base editing in cultured
mammalian cells… We provide guidelines for choosing target sites, appropriate base editor variants …

… To determine if either Cas9 or sgRNA expression is a limiting factor in the observed genome
editing reactions, lysates prepared from the transfected cells were incubated with plasmid …

CRISPR-Cas9 is quickly revolutionizing the way we approach gene therapy. CRISPR-Cas9
is a complexed, two-component system using a short guide RNA (gRNA) sequence to direct …

… , scalable, and cost-efficient genome editing in mammalian cells. … guide RNAs for targeted
genome editing only requires the … the SpCas9 system for genome editing in mammalian cells. …

… in the host cell. This article provides detailed methods for genome editing using the RNP …
using lipofection or electroporation in mammalian cells or using microinjection in murine …

… genome editing (37), but this system has not yet been applied to mammalian genome editing.
… Our findings suggest novel genome editing tools for eukaryotic cells using the CRISPR …

… ZFN-mediated genome editing in mammalian cells to create precise, in-frame fluorescent
protein fusions at the genomic loci of endocytic proteins. In yeast cells, where construction of …

… Here, we report our studies of genome editing in mammalian cells using the CRISPR-Cas9
system from Neisseria meningitidis (Nme) that has previously been demonstrated to …

We describe the development and application of a novel series of vectors that facilitate
CRISPR-Cas9-mediated genome editing in mammalian cells, which we call CRISPR-Bac. …

Programmable DNA nucleases such as TALENs and CRISPR/Cas9 are emerging as powerful
tools for genome editing. Dual-fluorescent surrogate systems have been demonstrated by …