Structural and functional characterization of SARS-CoV-2 RBD domains produced in mammalian cells

C Gstottner, T Zhang, A Resemann, S Ruben… - Analytical …, 2021 - ACS Publications
As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is still
ongoing and dramatically influences our life, the need for recombinant viral proteins for …

Human coronavirus EMC does not require the SARS-coronavirus receptor and maintains broad replicative capability in mammalian cell lines

MA Müller, VS Raj, D Muth, B Meyer, S Kallies… - MBio, 2012 - Am Soc Microbiol
… virus is related to SARS-CoV, we investigated whether it might replicate in bat cells and use
the … This knowledge is highly critical, because the SARS-CoV receptor influenced pathology, …

[HTML][HTML] Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells

Scientific reports, 2020 - nature.com
… In this work we expressed and purified SARS-CoV-2 Spike RBD from two different
systems—the yeast P. pastoris and HEK-293T mammalian cells—and compared their structure, …

[HTML][HTML] On the origin and evolution of SARS-CoV-2

D Singh, SV Yi - Experimental & molecular medicine, 2021 - nature.com
… The spike (S) protein of SARS-CoV-2, which enables the virus to enter host cells, exhibits …
and many other mammalian cells. The global spread and explosive growth of the SARS-CoV-2 …

[HTML][HTML] Recombinant protein expression and purification of N, S1, and RBD of SARS-CoV-2 from mammalian cells and their potential applications

J Garcia-Cordero, J Mendoza-Ramirez… - Diagnostics, 2021 - mdpi.com
coronavirus (SARS-CoV-2) are the structural proteins spike (S) protein and nucleocapsid (N)
protein. The spike protein of SARS-CoV-… proteins from SARS-CoV-2 in a mammalian system…

[HTML][HTML] Mechanisms of SARS-CoV-2 entry into cells

CB Jackson, M Farzan, B Chen, H Choe - … reviews Molecular cell …, 2022 - nature.com
… the structural and cellular foundations for understanding the multistep SARS-CoV-2 entry
process… , antibodies and other potential therapeutics targeting SARS-CoV-2 entry mechanisms. …

Synthetic antibodies neutralize SARS-CoV-2 infection of mammalian cells

S Miersch, M Ustav Jr, Z Li, JB Case, S Ganaie… - BioRxiv, 2020 - biorxiv.org
SARS-CoV-2 spike protein, compete for binding with ACE2, and potently inhibit infection by
SARS-CoV-… virus infection in Vero E6 cells, potently inhibiting authentic SARS-CoV-2 virus at …

[HTML][HTML] Hydroxychloroquine blocks SARS-CoV-2 entry into the endocytic pathway in mammalian cell culture

Z Yuan, MA Pavel, H Wang, JC Kwachukwu… - Communications …, 2022 - nature.com
… as a treatment for SARS-coronavirus-2 (SARS-CoV-2) infection… These same lipid clusters
recruit the SARS-CoV-2 surface … resolution imaging of cultured mammalian cells (VeroE6, A549…

[HTML][HTML] Interaction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems

JY Chen, WN Chen, KMV Poon, BJ Zheng, X Lin… - Archives of …, 2009 - Springer
cellular factors involved in SARS coronavirus replication, the helicase (Hel, nsp13) of SARS
coronavirus was used to screen the cDNA library of rat pulmonary epithelial cells using the …

SARSCoV‐2 replicating in nonprimate mammalian cells probably have critical advantages for COVID‐19 vaccines due to anti‐Gal antibodies: a minireview and …

JM Chen - Journal of Medical Virology, 2021 - Wiley Online Library
… This review suggests with evidence that SARS-CoV-2 replicating in nonprimate mammalian
cells, if employed as live or inactivated vaccines, may induce higher immunity against COVID…