Structural and functional characterization of SARS-CoV-2 RBD domains produced in mammalian cells
C Gstottner, T Zhang, A Resemann, S Ruben… - Analytical …, 2021 - ACS Publications
As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is still
ongoing and dramatically influences our life, the need for recombinant viral proteins for …
ongoing and dramatically influences our life, the need for recombinant viral proteins for …
Human coronavirus EMC does not require the SARS-coronavirus receptor and maintains broad replicative capability in mammalian cell lines
… virus is related to SARS-CoV, we investigated whether it might replicate in bat cells and use
the … This knowledge is highly critical, because the SARS-CoV receptor influenced pathology, …
the … This knowledge is highly critical, because the SARS-CoV receptor influenced pathology, …
[HTML][HTML] Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells
Scientific reports, 2020 - nature.com
… In this work we expressed and purified SARS-CoV-2 Spike RBD from two different
systems—the yeast P. pastoris and HEK-293T mammalian cells—and compared their structure, …
systems—the yeast P. pastoris and HEK-293T mammalian cells—and compared their structure, …
[HTML][HTML] On the origin and evolution of SARS-CoV-2
… The spike (S) protein of SARS-CoV-2, which enables the virus to enter host cells, exhibits …
and many other mammalian cells. The global spread and explosive growth of the SARS-CoV-2 …
and many other mammalian cells. The global spread and explosive growth of the SARS-CoV-2 …
[HTML][HTML] Recombinant protein expression and purification of N, S1, and RBD of SARS-CoV-2 from mammalian cells and their potential applications
J Garcia-Cordero, J Mendoza-Ramirez… - Diagnostics, 2021 - mdpi.com
… coronavirus (SARS-CoV-2) are the structural proteins spike (S) protein and nucleocapsid (N)
protein. The spike protein of SARS-CoV-… proteins from SARS-CoV-2 in a mammalian system…
protein. The spike protein of SARS-CoV-… proteins from SARS-CoV-2 in a mammalian system…
[HTML][HTML] Mechanisms of SARS-CoV-2 entry into cells
… the structural and cellular foundations for understanding the multistep SARS-CoV-2 entry
process… , antibodies and other potential therapeutics targeting SARS-CoV-2 entry mechanisms. …
process… , antibodies and other potential therapeutics targeting SARS-CoV-2 entry mechanisms. …
Synthetic antibodies neutralize SARS-CoV-2 infection of mammalian cells
… SARS-CoV-2 spike protein, compete for binding with ACE2, and potently inhibit infection by
SARS-CoV-… virus infection in Vero E6 cells, potently inhibiting authentic SARS-CoV-2 virus at …
SARS-CoV-… virus infection in Vero E6 cells, potently inhibiting authentic SARS-CoV-2 virus at …
[HTML][HTML] Hydroxychloroquine blocks SARS-CoV-2 entry into the endocytic pathway in mammalian cell culture
… as a treatment for SARS-coronavirus-2 (SARS-CoV-2) infection… These same lipid clusters
recruit the SARS-CoV-2 surface … resolution imaging of cultured mammalian cells (VeroE6, A549…
recruit the SARS-CoV-2 surface … resolution imaging of cultured mammalian cells (VeroE6, A549…
[HTML][HTML] Interaction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems
JY Chen, WN Chen, KMV Poon, BJ Zheng, X Lin… - Archives of …, 2009 - Springer
… cellular factors involved in SARS coronavirus replication, the helicase (Hel, nsp13) of SARS
coronavirus was used to screen the cDNA library of rat pulmonary epithelial cells using the …
coronavirus was used to screen the cDNA library of rat pulmonary epithelial cells using the …
SARS‐CoV‐2 replicating in nonprimate mammalian cells probably have critical advantages for COVID‐19 vaccines due to anti‐Gal antibodies: a minireview and …
JM Chen - Journal of Medical Virology, 2021 - Wiley Online Library
… This review suggests with evidence that SARS-CoV-2 replicating in nonprimate mammalian
cells, if employed as live or inactivated vaccines, may induce higher immunity against COVID…
cells, if employed as live or inactivated vaccines, may induce higher immunity against COVID…
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