Overview of vector design for mammalian gene expression
RJ Kaufman - Molecular biotechnology, 2000 - Springer
… Mammalian cells are used as a host for gene expression in order to: … expression in
mammalian cells. This article reviews vector design for expression of foreign genes in mammalian …
mammalian cells. This article reviews vector design for expression of foreign genes in mammalian …
Overview of vector design for mammalian gene expression
RJ Kaufman - Recombinant Gene Expression Protocols, 1997 - Springer
… The technology of expressing foreign genes in mammalian cells has become increasingly
… Mammalian cells are frequently used as a host for expression of foreign genes because: …
… Mammalian cells are frequently used as a host for expression of foreign genes because: …
Recombinant adeno-associated virus vector design and gene expression in the mammalian brain
JC Paterna, H Büeler - Methods, 2002 - Elsevier
… gene expression within the mammalian brain are determined by several factors. These include
the dose of infectious particles, the purity of the vector stock, … in rAAV vector design is of …
the dose of infectious particles, the purity of the vector stock, … in rAAV vector design is of …
Vectors for gene expression in mammalian cells
SC Makrides - New comprehensive biochemistry, 2003 - Elsevier
… gene expression in several cell lines [22]. The use of introns, however, demands careful
vector design, as it is possible that cryptic splicing signals may cause aberrant processing of the …
vector design, as it is possible that cryptic splicing signals may cause aberrant processing of the …
Multi-chromatic control of mammalian gene expression and signaling
K Müller, R Engesser, S Schulz, T Steinberg… - Nucleic acids …, 2013 - academic.oup.com
… Given the previous success of chemically triggered mammalian gene switches in designing
synthetic gene networks and developing biomedical applications, we are convinced that the …
synthetic gene networks and developing biomedical applications, we are convinced that the …
De novo design and construction of an inducible gene expression system in mammalian cells
M Karlsson, W Weber, M Fussenegger - Methods in enzymology, 2011 - Elsevier
… basis of inducible expression systems in mammalian cells is … gene expression in mammalian
cells, two principal designs … In the activation-based system (Fig. 11.1A), the prokaryotic …
cells, two principal designs … In the activation-based system (Fig. 11.1A), the prokaryotic …
Optimization of protein expression in mammalian cells
M Hunter, P Yuan, D Vavilala… - Current protocols in …, 2019 - Wiley Online Library
… Modular vector designs utilizing common cloning sites allow different vector design strategies
to mix … Many examples exist demonstrating the impact of codon usage on gene expression. …
to mix … Many examples exist demonstrating the impact of codon usage on gene expression. …
Design and construction of targeted AAVP vectors for mammalian cell transduction
A Hajitou, R Rangel, M Trepel, S Soghomonyan… - Nature protocols, 2007 - nature.com
… vectors for mammalian cells, we reasoned that the genetic incorporation of compatible cis-elements
(such as inverted terminal repeats (ITRs)) from a mammalian—yet … gene expression …
(such as inverted terminal repeats (ITRs)) from a mammalian—yet … gene expression …
[HTML][HTML] How to choose the right inducible gene expression system for mammalian studies?
… , temporarily and quantitively at will, depending on experimental requirements and
designs. Here, we review a number of most commonly used mammalian inducible expression …
designs. Here, we review a number of most commonly used mammalian inducible expression …
A comparative study of different vector designs for the mammalian expression of recombinant IgG antibodies
… a series of mammalian expression vectors for the production of … antibodies with different
expression cassette designs. Versions … that expression of the downstream YFP gene expression …
expression cassette designs. Versions … that expression of the downstream YFP gene expression …
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