… One bottleneck in membrane protein structural biology pipelines is the low abundance of
the membrane protein of interest. Membrane proteins are naturally expressed at low levels (…

… In combination, these diverse approaches provide a technical platform for overcoming the
major bottlenecks in membrane protein structural biology. This potential to build on recent …

… When solubilized in detergents, many membrane proteins cannot be … bottleneck is particularly
acute for membrane proteins from higher eukaryotes, and most membrane proteins whose …

… to alleviate cellular limitations during microbial membrane protein expression. Lately, …
bottlenecks during heterologous protein production and their use in microbial membrane protein …

… Tate and coworkers reported a positive effect of calnexin and calreticulin overexpression
on recombinant membrane proteins in insect cells (87, 183), while a two- to threefold increase …

… of the membrane protein structures in the protein databank were … a major bottleneck for
challenging membrane proteins, … efficient approaches are needed to overcome this bottleneck. …

… overcome important bottlenecks such as folding and the rate of translocation between cell
compartments. Especially in membrane protein … and immediate rate of protein-specific mRNA …

… Whereas glucose consumption rarely presents a bottleneck for … to bioinformatically identify
membrane proteins of high biotech … to overcome transport flux bottlenecks in S. cerevisiae. …

… control systems also represent significant bottlenecks in heterologous protein production [18,
19]… To overcome some of these difficulties new methods for over-expression of two or more …

… and/or are integral membrane proteins are often difficult to … protein yields (Table 1) that can
overcome bottlenecks in … cases that target potential bottlenecks in protein production, using …