Molecular characterization of porcine picobirnaviruses and development of a specific reverse transcription-PCR assay
GM Carruyo, G Mateu, LC Martínez… - Journal of Clinical …, 2008 - Am Soc Microbiol
Journal of Clinical Microbiology, 2008•Am Soc Microbiol
The molecular characterization of partial-length genomic segment 2 of porcine
picobirnavirus (PBV) strains and the development of a specific reverse transcription-PCR
(RT-PCR) assay for detection of virus in feces are reported. Phylogenetic analysis indicated
that the studied porcine isolates were more closely related (> 85% identity) to human PBV
belonging to genogroup I than to the other porcine PBV described so far. Analysis by RT-
PCR and polyacrylamide gel electrophoresis of fecal samples collected in Venezuela and …
picobirnavirus (PBV) strains and the development of a specific reverse transcription-PCR
(RT-PCR) assay for detection of virus in feces are reported. Phylogenetic analysis indicated
that the studied porcine isolates were more closely related (> 85% identity) to human PBV
belonging to genogroup I than to the other porcine PBV described so far. Analysis by RT-
PCR and polyacrylamide gel electrophoresis of fecal samples collected in Venezuela and …
Abstract
The molecular characterization of partial- length genomic segment 2 of porcine picobirnavirus (PBV) strains and the development of a specific reverse transcription-PCR (RT-PCR) assay for detection of virus in feces are reported. Phylogenetic analysis indicated that the studied porcine isolates were more closely related (>85% identity) to human PBV belonging to genogroup I than to the other porcine PBV described so far. Analysis by RT-PCR and polyacrylamide gel electrophoresis of fecal samples collected in Venezuela and Argentina showed that PBV circulate at high frequencies in piglets.
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