A monoclonal antibody (MAb), EM-7G1, specific for Listeria monocytogenes was developed
by using a previously developed MAb, C11E9 (AK Bhunia, PH Ball, AT Fuad, BW Kurz, JW
Emerson, and MG Johnson, Infect. Immun. 59: 3176-3184, 1991), to mask epitopes shared
by L. monocytogenes and Listeria innocua in a 66-kDa cell surface protein. MAb EM-7G1
was an immunoglobulin subclass G1 antibody with kappa light chains. This MAb reacted
with all 34 strains of L. monocytogenes tested and showed no cross-reaction with other …

Eight hundred fifty-nine murine hybridomas were produced from eight fusions, and 27 were
characterized for secretion of antibodies reactive to Listeria monocytogenes. One
monoclonal antibody (MAb), P5C9, reacted with all test strains of L. monocytogenes (31 of
31), L. innocua (3 of 3), and L. welshimeri (1 of 1) but not with any strains of the other four
Listeria species or with any of 22 gram-positive or 11 gram-negative species of bacteria
when tested in microtiter and dot blot enzyme immunoassays. Of the other 26 antibodies, 20 …