The TOL network of Pseudomonas putida mt2 processes multiple environmental inputs into a narrow response space

R Silva‐Rocha, V de Lorenzo - Environmental microbiology, 2013 - Wiley Online Library
… of network attractors (Bornholdt, 2008). This analysis allowed us to identify and assess the
TOL … We discovered that the inner logic structure of the TOL network restricts the expression of …

Increasing Signal Specificity of the TOL Network of Pseudomonas putida mt-2 by Rewiring the Connectivity of the Master Regulator XylR

A de Las Heras, S Fraile, V de Lorenzo - 2012 - journals.plos.org
… In this work, we report one strategy to overcome the constraints imposed by the natural
architecture of the TOL network on the function of the XylR/Pu regulatory node of the plasmid. To …

Decoding the genetic networks of environmental bacteria: regulatory moonlighting of the TOL system of Pseudomonas putida mt-2

R Silva-Rocha, D Pérez-Pantoja… - The ISME journal, 2013 - academic.oup.com
… (b) The extant TOL network of P. putida mt-2 for metabolization of m-xylene. The system has
two operons: upper and lower, expressed from the Pu and Pm promoters, respectively. The …

The regulatory logic of mxylene biodegradation by Pseudomonas putida mt2 exposed by dynamic modelling of the principal node Ps/Pr of the TOL plasmid

M Koutinas, MC Lam, A Kiparissides… - Environmental …, 2010 - Wiley Online Library
… control network of the TOL plasmid pWW0 born by Pseudomonas putida mt-2 for biodegradation
of m-… In order to penetrate the underlying logic of such a network, which controls a major …

Inferring the genetic network of mxylene metabolism through expression profiling of the xyl genes of Pseudomonas putida mt2

F Velázquez, V Parro, V de Lorenzo - Molecular microbiology, 2005 - Wiley Online Library
… of the TOL plasmid pWW0 of Pseudomonas putida mt-2 has … induction of the meta TOL
operon by m-xylene needs the build-… putida mt-2 to direct addition of m-toluate. This allows the …

High‐resolution analysis of the m‐xylene/toluene biodegradation subtranscriptome of Pseudomonas putida mt2

J Kim, D Pérez‐Pantoja, R Silva‐Rocha… - Environmental …, 2016 - Wiley Online Library
… Response of the TOL network to gratuitous induction and to 3MBz To complete the picture
of the response of the TOL network to its canonical substrate, m-xylene, we examined the …

Subcellular Architecture of the xyl Gene Expression Flow of the TOL Catabolic Plasmid of Pseudomonas putida mt-2

J Kim, A Goñi-Moreno, V de Lorenzo - Mbio, 2021 - Am Soc Microbiol
… metabolic and regulatory components of the TOL network and visualization of transcripts
from either the upper or the lower operons of the system. (A) In the presence of m-xylene, XylR (…

Stochasticity of TOL plasmid catabolic promoters sets a bimodal expression regime in Pseudomonas putida mt2 exposed to mxylene

R Silva‐Rocha, V de Lorenzo - Molecular microbiology, 2012 - Wiley Online Library
… bacteria confront m-xylene only. We make the case that this is not a casual occurrence but
that such performance encoded in the TOL network optimizes the adaptation of P. putida mt-2

Improving the prediction of Pseudomonas putida mt-2 growth kinetics with the use of a gene expression regulation model of the TOL plasmid

M Koutinas, A Kiparissides, MC Lam… - Biochemical …, 2011 - Elsevier
… of the TOL (pWW0) plasmid of Pseudomonas putida mt-2, involved in the metabolism of m-xylene,
with the growth kinetics of the microorganism to predict the biodegradation of m-…

The logic layout of the TOL network of Pseudomonas putida pWW0 plasmid stems from a metabolic amplifier motif (MAM) that optimizes biodegradation of m-xylene

R Silva-Rocha, H de Jong, J Tamames… - BMC Systems Biology, 2011 - Springer
network of the TOL plasmid pWW0 of the soil bacterium Pseudomonas putida mt-2 for
catabolism of m-… To gain an insight into the inner layout of this network a logic model of the TOL