Peripheral blood marker of residual acute leukemia after hematopoietic cell transplantation using multi-plex digital droplet PCR

M Stanojevic, M Grant, SK Vesely, S Knoblach… - Frontiers in …, 2022 - frontiersin.org
… from mononuclear cells from acute leukemia patients after HCT (n = 31 … measured in patients
with and without active leukemia after HCT. … We therefore developed a multiplex ddPCR to …

Novel Multiplex Droplet Digital PCR Assays to Monitor Minimal Residual Disease in Chronic Myeloid Leukemia Patients Showing Atypical BCR-ABL1 Transcripts

J Petiti, M Lo Iacono, M Dragani, L Pironi… - Journal of clinical …, 2020 - mdpi.com
… marker in chronic myeloid leukemia; 2% of patients show unusual breakpoints generating …
patients’ molecular remission, excluding them from treatment-free-remission protocols. Droplet

Partitioning for Easy Multiplexing: A Versatile Droplet PCR Application for Clone Monitoring in Tumors

A Kinoo, A Caye-Eude, Y Vial, H Cavé… - The Journal of Molecular …, 2023 - Elsevier
… cfDNA was also obtained from a leukemia patient's peripheral blood collected on a Cell-Free
DNA Collection Tube (Roche, Basel, Switzerland). After centrifugation, cfDNA was isolated …

Digital droplet PCR in hematologic malignancies: a new useful molecular tool

S Galimberti, S Balducci, F Guerrini, M Del Re… - Diagnostics, 2022 - mdpi.com
… Italian group set a multiplexing ddPCR in patients affected by chronic myeloid leukemia (CML)
with … a new kind of “higher order multiplexing ddPCR” able to simultaneously measure the …

Multiplex droplet digital PCR assay for quantification of human T-cell leukemia virus type 1 subtype c DNA proviral load and T cells from blood and respiratory …

D Yurick, G Khoury, B Clemens, L Loh… - Journal of clinical …, 2019 - Am Soc Microbiol
… a QX-200 droplet generator, droplets were … Droplets were analyzed with a QX200 droplet
reader (Bio-Rad) using a two-channel setting to detect FAM and HEX. The positive droplets

High-throughput single-cell DNA sequencing of acute myeloid leukemia tumors with droplet microfluidics

M Pellegrino, A Sciambi, S Treusch… - Genome …, 2018 - genome.cshlp.org
… of individual cancer cells confined to droplets. The barcodes are … myeloid leukemia (AML)
tumor populations from two patientsDroplet PCR reactions consisted of 1× platinum multiplex

Quantification of BCR-ABL1 Fusion Transcripts in Patients With Acute B Lymphoblastic Leukemia by Multiplex Droplet Digital PCR

R Martinez, D Nennig, P Mroz - American Journal of Clinical …, 2019 - academic.oup.com
… , multiplexed ddPCR assay for detecting BCR-ABL1 e1a2 and ABL1 e10 from RNA. Droplets
were prepared using a QX100 or QX200 droplet … on a QX100 droplet reader and analyzed …

[HTML][HTML] Minimal residual disease monitoring of acute myeloid leukemia by massively multiplex digital PCR in patients with NPM1 mutations

N Mencia-Trinchant, Y Hu, MA Alas, F Ali… - The Journal of Molecular …, 2017 - Elsevier
… primer, multiplex reverse primer, … droplets of type-specific, multiplex, and deoxyinosine-based
NPM1 mutation assays in type A NPM1 patient samples (NPM1mut acute myeloid leukemia

One-Step Multiplexed Droplet Digital Polymerase Chain Reaction for Quantification of p190 BCR-ABL1 Fusion Transcript in B-Lymphoblastic Leukemia

RJ Martinez, Q Kang, D Nennig… - … of Pathology & …, 2022 - meridian.allenpress.com
… monitoring in patients with acute B-lymphoblastic leukemia. Droplet digital polymerase chain
… to generate individual reaction chambers (droplets) in which individual amplification targets …

Multiplex droplet digital PCR quantification of recurrent somatic mutations in diffuse large B-cell and follicular lymphoma

M Alcaide, S Yu, K Bushell, D Fornika… - Clinical …, 2016 - academic.oup.com
… and 13 across a cohort of plasma or FFPE samples obtained from colorectal cancer patients.
Other multiplexed digital PCR designs have recently permitted researchers, for instance, to …