Comparison of four multiplex PCR assays for the detection of viral pathogens in respiratory specimens

TP Anderson, AM Werno, K Barratt… - Journal of Virological …, 2013 - Elsevier
… However, there are few direct comparisons of different PCR assays. This study compares 4
different multiplex PCR assays for the recovery of common respiratory viruses. We tested 213 …

Evaluation of a multiplexed PCR assay for detection of respiratory viral pathogens in a public health laboratory setting

DJ Marshall, E Reisdorf, G Harms, E Beaty… - Journal of clinical …, 2007 - Am Soc Microbiol
… The PLx-RVP assay is a multiplexed molecular diagnostic system that was formulated for
this study to detect 17 respiratory-pathogen targets simultaneously. The assay took …

Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses

G Boivin, S Côté, P Déry, G De Serres… - Journal of clinical …, 2004 - Am Soc Microbiol
… % for the antigenic test. In conclusion, our multiplex real-time PCR assay combines both
rapidity and sensitivity for detecting the most important respiratory viral pathogens in children. …

Rapid multiplex nested PCR for detection of respiratory viruses

WY Lam, ACM Yeung, JW Tang, M Ip… - Journal of clinical …, 2007 - Am Soc Microbiol
… This study developed and evaluated five groups of multiplex nested PCR assays that could
simultaneously detect 21 different respiratory pathogens: influenza A virus (H1N1, H3N2, …

Multiplexed reverse transcriptase PCR assay for identification of viral respiratory pathogens at the point of care

SE Létant, JI Ortiz, LF Bentley Tammero… - Journal of clinical …, 2007 - Am Soc Microbiol
assay performance in terms of sensitivity and specificity. The limits of detection are provided
for each targeted respiratory pathogen… specificity of the multiplexed assay to the combination …

Multiplex PCR and emerging technologies for the detection of respiratory pathogens

AM Caliendo - Clinical infectious diseases, 2011 - academic.oup.com
… from a single test. The RespPlex I test is designed to detect bacterial respiratory pathogens,
… varied from 72% to 100% when compared with culture and monoplex real-time PCR assays, …

Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested‐PCR assays

MT Coiras, JC Aguilar, ML Garcia… - Journal of medical …, 2004 - Wiley Online Library
… across the range of viral pathogens [Myint, 2002]. For many RNA viruses, including most of
respiratory viruses, the multiplex reverse transcription (RT)-PCR assay-based diagnosis has …

Identification of viral and atypical bacterial pathogens in children hospitalized with acute respiratory infections in Hong Kong by multiplex PCR assays

RYT Sung, PKS Chan, T Tsen, AM Li… - Journal of medical …, 2009 - Wiley Online Library
… used multiplex PCR assays to assess the role of 20 respiratory viruses and atypical bacteria
as a cause of hospital admission for children with suspected acute respiratory infections in …

Impact of early detection of respiratory viruses by multiplex PCR assay on clinical outcomes in adult patients

U Rappo, AN Schuetz, SG Jenkins… - Journal of clinical …, 2016 - Am Soc Microbiol
… multiple viral pathogens with a single test (4). The FilmArray respiratory viral panel (RVP)
was FDA cleared in May 2011 to detect 15 respiratory viral pathogens: influenza A virus and …

Comparative evaluation of six commercialized multiplex PCR kits for the diagnosis of respiratory infections

S Pillet, M Lardeux, J Dina, F Grattard, P Verhoeven… - PloS one, 2013 - journals.plos.org
… explain some missed results with the multiplex PCR assays tested in this study; indeed, this
technology favors primers’ competition, notably for the pathogen(s) exhibiting the lower load …