… deep mutational scanning to determine how all amino acid mutations to the SARS-CoV-2
RBD … of the RBD using a PCR-based mutagenesis method that introduces all 19 mutant amino …

… Illumina sequencing, the mutagenic library was divided into 2 … prepared by separate nicking
mutagenesis reactions. Library … To screen the core residue mutagenic library for protease-…

… Furthermore, mutagenesis described in this study was performed in the S2 region instead
of the immunodominant RBD in the S1 subunit. As a result, most people should have robust …

… scanning, which combines saturation mutagenesis and next-generation sequencing (30).
Previous studies have applied deep mutational scanning … Although deep mutational scanning …

… This study uses deep mutagenesis scanning with whole trimeric spike displayed on
mammalian cells to screen for mutations that increase ACE2 binding and escape antibodies. We …

… Deep mutagenesis or deep mutational scanning take advantage of next-generation …
prominent studies of SARS coronavirus 2 (SARS-CoV-2) have used deep mutagenesis to address …

… mutagenesis-based remodeling of SARs-CoV-1 peptide (ATLQAIAS) was performed to inhibit
the activities of 3CLpro of SARS-CoV… this alanine scanning mutagenesis and residue scan …

… domain of SARS-CoV-2 (S-RBD CoV-2 ) showing the hot-spot residues at the protein/protein
binding interface as identified in our previous computational alanine scanning study. (25) …

… The fusion subunit of the SARS-CoV S glycoprotein contains two regions of hydrophobic …
serine-scanning mutagenesis to the C-terminal-most heptad-repeat region in the SARS-CoV S …

… Experimental epitope mapping approaches use structure determination, site-directed
mutagenesis such as alanine-scanning, peptide arrays, and/or mass spectrometry. Each technique …