… view of the antigenic sites on the SARS-CoV-2 RBD and the determinants of RBD NAb
recognition, we performed global RBD alanine scanning mutagenesis (at nearly 190 RBD amino …

… scanning results of the spike protein RBD of SARS-CoV-1 … In summary, alanine scans
indicate that SARS-CoV-2 has … with the exhaustive mutagenesis study on SARS-CoV-2 RBD …

… IMPORTANCE The spike protein of SARS-CoV-2 is a major … The results of our study
demonstrate that SARS-CoV-2 rapidly … Thus, the evolved SARS-CoV-2 variants efficiently use …

… Control of the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
pandemic will require deployment of multiple countermeasures, including therapeutics and …

… a deep mutational scanning approach to … the SARS-CoV-2 isolate Wuhan-Hu-1 (Genbank
accession number MN908947, residues N331-T531) via NNS codon tiling PCR mutagenesis, …

… The cellular entry of SARS-CoV-2 is primarily mediated by binding of the receptor binding
… Briefly, all these plasmids were generated by a QuikChange II XL site-directed mutagenesis …

… mutagenesis analysis reported here will guide further experimental studies of Ab resistance
and identification of natural SARS-CoV… the complete virtual mutation scanning of RBD (…

… A comparative analysis between SARS-CoV and SARS-CoV-2 is offered below. … alanine
scanning mutagenesis We next used BAlaS, 9 a computational alanine scanning mutagenesis …

… were scanned for 4 min at 90 kV, 88 μA, FOV 45 mm and a pixel size 90.0 μm. After scanning,
the … -2 S D614G/P681R mutant was generated by site-directed mutagenesis PCR using pC-…

… SARS-CoV-2 S binding with a panel of nanobodies that agrees with a wide spectrum of
mutagenesis … We conducted mutational scanning analysis of the binding epitope residues for the …