… This study uses deep mutagenesis scanning with whole trimeric spike displayed on
mammalian cells to screen for mutations that increase ACE2 binding and escape antibodies. We …

… Phenotypes of numerous mutations … scanning, which combines saturation mutagenesis
and next-generation sequencing (30). Previous studies have applied deep mutational scanning …

… Finally, we show that the spike phenotypes we measure explain much of the changes in viral
… For both XBB.1.5 full spike and RBD-only libraries we pooled spike mutagenesis primers at …

… To check whether this phenotype was the result of the charge of the lysine, an alanine was …
Lysine scanning mutagenesis Mutagenesis by lysine insertion showed that at position 1201 a …

… scanning, which combines saturation mutagenesis and next-generation sequencing, allows
the phenotypes … Furthermore, mutagenesis described in this study was performed in the S2 …

… glycoprotein, we first established a lentiviral pseudotyping platform that maintains a
genotype-phenotype link between the lentiviral genome and the spike variant on the virion’s surface. …

… We previously used deep mutational scanning to … We constructed comprehensive site-saturation
mutagenesis … the Omicron BA.1 spike reduces cell entry by spike-pseudotyped lentiviral …

… tolerance within the spike that may have … spike and its receptor, the underlying selection
strategies used in deep mutational scans are increasingly tied to more complex phenotypes…

The receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein mediates viral
attachment to ACE2 receptor and is a major determinant of host range and a dominant target …

… Differential scanning fluorimetry revealed that both 2P and 2PQ had an apparent melting …
site-directed mutagenesis. Pairs of primers used for PCR-based site directed mutagenesis are …