… typhimurium and derivative strains overexpressing either the needle protein PrgI or the
inner rod protein PrgJ. Values are the means ± standard deviation of three independent …

… of the Salmonella inner rod protein subunit PrgJ we present evidence that the assembly of
the inner rod dictates the timing of substrate switching and needle length. Furthermore, the …

… of secretion of the inner-rod and needle proteins, thus determining the length of the … evidence
that needle length is not determined by the rate of inner rod versus needle polymerization, …

… needle that delivers virulence effectors into host cells. Here, we showed that the Yersinia inner
rod protein YscI directly interacts with the needle protein … as well as needle assembly and …

… protein assemblies has not yet been achieved. Here, the 2D assembly pattern of protein
needles (… The PN is an anisotropic needle‐shaped protein composed of β‐helix, foldon, and His‐…

… pestis, the needle length is defined by the protein ruler YscP (3), and the proteins injected
… to 319 within the coiled-coil rod, yielding shorter (YadA 365 , estimated length of 15 nm) and …

… of YscP suffices to control the length of one needle or whether several molecules of YscP …
needle subunit YscF until the needle length matches the ruler length, which would stop needle …

… The rings, which are ≈40 nm in diameter at their widest section, are joined by a central rod
and together form the base of the needle complex. Protruding outward from the base is the …

… The inner rod protein SctI is a homolog of the needle filament protein SctF with an identity
of 24% in the T3SS encoded on Salmonella Typhimurium pathogenicity island 1 (SPI-1). …

… of two YscP mutant proteins of different length in vivo led to … needle length matching either
mutant protein. This supported the model that a single ruler protein regulates needle length in …