… that real-time RT-PCR, when normalized for the total ABL transcripts, can be used to … nested,
competitive RT-PCR be used to determine BCR-ABL/ABL transcript ratios at low transcript …

… ALL for the presence of underlying BCR-ABL gene fusions. Positive and … by nested PCR.
We studied 21 patients for detecting the presence of b3a2, b2a2 and ela2 BCR-ABL transcripts …

… ) is a powerful and rapid method for specifically detecting BCR–ABL … reverse transcription
of BCR–ABL mRNA. We studied 29 patients for detecting the presence of BCR-ABL transcripts …

… BCR-ABL fusion transcript. Two samples with a BCR-ABL transcript in the first RT-nested-PCR
approach yielded another transcript in the second RT-PCR that was consistently obtained …

… of multiplex-PCR in the specific detection of BCR-ABL transcripts in different hematological
… to nested PCR. Fifty-three patients were studied for the presence of BCR-ABL transcripts: 24 …

… PCR (external primers A ↔ B) and nested PCR (… PCR analyses, whereas the nested
PCR analyses generally reached the minimal target sensitivity of 10−4. The standardized RT-PCR …

… We sought evidence of BCR/ABL transcripts in the peripheral … reaction (PCR) using (a) a
single-stage PCR comprising 30 … was subjected to a further 30 cycles with nested amplimers. …

… sensitivity of RT-qPCR might be increased by modifications such as performing nested PCR,
increasing the amount of RNA assayed, or increasing the efficiency of reverse transcription. …

… to ABL exon 2 were designed, enabling detection of the most frequent bcr-abl transcripts, and
also of the normal ABL-Ia transcript … as high as standard nested RT-PCR, and significantly …

… To simplify this procedure we have developed a nested, multiplex RT-PCR assay in which
… p210 bcr-abl transcripts and [32 microglobulin) are mixed simultaneously in the initial PCR …