Multidimensional solid-state NMR spectroscopy of plant cell walls

T Wang, P Phyo, M Hong - Solid state nuclear magnetic resonance, 2016 - Elsevier
… A better understanding of the plant cell wall structure on the … cell wall mass per hectare,
how to reduce cell wall recalcitrance to enzymatic degradation, and how to optimize cell wall

Characterization of nonderivatized plant cell walls using high‐resolution solution‐state NMR spectroscopy

DJ Yelle, J Ralph, CR Frihart - Magnetic Resonance in …, 2008 - Wiley Online Library
… whole cell wall, without derivatization, in suitable NMR solvents. Again, if the whole cell wall
is … characterized via solution-state NMR. The DMSO/NMI system will fully dissolve the whole …

Investigation of the action patterns of pectinmethylesterase isoforms through kinetic analyses and NMR spectroscopy: implications in cell wall expansion

L Catoire, M Pierron, C Morvan, CH Du Penhoat… - Journal of Biological …, 1998 - ASBMB
… From the behavior of solubilized and cell wall-bound PMEs, we ultimately aimed to shed
light on the role of the different PME isoforms in the cell wall-stiffening process, which occurs …

In-cell NMR spectroscopy

Z Serber, L Corsini, F Durst, V Dötsch - Methods in enzymology, 2005 - Elsevier
NMR spectra. In this chapter, we will concentrate only on the application of NMR spectroscopy
to macromolecules in living cells … possess a very robust cell wall, and Xenopus oocytes, …

Solid-state NMR of plant and fungal cell walls: a critical review

W Zhao, LD Fernando, A Kirui, F Deligey… - … magnetic resonance, 2020 - Elsevier
… solid-state NMR spectroscopy has been extensively employed to investigate intact cell walls.
… spectra collected on whole cells or isolated cell walls provide the atomic resolution needed …

Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR

JAH Romaniuk, L Cegelski - … Transactions of the Royal …, 2015 - royalsocietypublishing.org
Cell wall yields by lyophilized dry mass of cell walls with respect to whole cells were 16%
for cells harvested at OD 660 0.7, 18% at OD 660 2.0 and 21% at OD 660 4.0. These harvests …

Assessment of cell wall porosity in Arabidopsis thaliana by NMR spectroscopy

C Rondeau-Mouro, D Defer, E Leboeuf… - International Journal of …, 2008 - Elsevier
… Wild type and mutant stems and calli cell walls were isolated and depleted in … cell walls,
respectively. Porosity in the different wall preparations was assessed by NMR spectroscopy. This …

Plant cell-wall cross-links by REDOR NMR spectroscopy

L Cegelski, RD O'Connor, D Stueber… - Journal of the …, 2010 - ACS Publications
… soybean cells and cell walls used in this study revealed these anticipated attributes (Table
1). Most hydroxyproline residues are in the cell wall. Glycine is more abundant in cell walls, …

Direct Observation of Cell Wall Structure in Living Plant Tissues by Solid-State 13C NMR Spectroscopy

MC Jarvis, DC Apperley - Plant physiology, 1990 - academic.oup.com
… All these tissues had thickened cell walls which allowed them to withstand the centrifugal …
NMR spectra. The celery collenchyma cell walls gave spectra typical of dicot primary cell walls. …

Whole plant cell wall characterization using solution-state 2D NMR

SD Mansfield, H Kim, F Lu, J Ralph - Nature protocols, 2012 - nature.com
… This paper describes the use of state-of-the-art NMR analysis of whole plant cell walls, and
… 2D NMR on two types of cell wall samples: acetylated cell walls and native cell walls. In both …