… for nuclear envelope assembly in Xenopus egg extract. However, in this context it has to be
kept in mind that demembranated Xenopus … to bind nascent nuclear membrane vesicles. The …

… Incubation of demembranated sperm in fractions of Xenopus egg extracts. Sperm were
incubated for 60 mill at 18C in S20o alone (A) or $20o plus NEP-A (B). In $20o alone vesicles …

… that annulate lamellae neither exist in intact Xenopus eggs nor in the initial egg extract. …
selected the mAb PI1 which has been shown to react with a nuclear pore complex glycopro- …

… this is coordinated with nuclear pore complex (NPC) assembly. … Xenopus laevis egg extracts
is mediated by SNARE proteins that require activation by NSF. Antibodies against Xenopus …

… of the extract can be easily manipulated. Here we describe how to assemble nuclei in Xenopus
egg extract, … , and different approaches to altering nucleocytoplasmic transport in extract. …

… Xenopus egg extract, sperm chromatin substrate, and applications using the … nuclear
envelope and a second protocol for analyzing nuclear pore complex assembly into preformed, pore-…

… Remarkably, incubation of egg extract with DNA in the presence of an energy … nuclear
envelope around DNA. In addition, these in vitro nuclei contain functional nuclear pore complexes, …

… We cannot exclude that a considerable number of pore complex proteins are extracted by
… pore complex architecture are maintained. The pore complex and probably also the nuclear …

… have been made by the use of Xenopus egg extracts which are able to assemble the NE in
… or at the terminal ring of the nuclear baskets of Xenopus oocyte NEs. Analysis of the primary …

… nuclear pore complex protein p68 is stored in Xenopus laevis eggs and its involvement in
pore complex … ) we removed this protein from Xenopus egg extract by immunoadsorption. On …