… We applied the hapten digoxigenin to the enzymatic labeling of oligonucleotides. This approach
… and RNA labeling systems based on digoxigenin incorporation (24,25,43,53,54). In the …

… oligonucleotide. In this study, we compared OPs labeled with multiple detectable haptens
(such as biotin, digoxigenin… to 64 haptens per OP and show that in cells, 16-32 haptens per OP …

… drug, digoxigenin acted as a hapten, and the humanized anti-digoxigenin antibody served
… degradation, aptamers and oligonucleotides show impaired in vivo functionality. To improve …

… Nonradioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing
with terminal transferase. Anal Biochem 1991;192:222-31. Coates PJ, Hall PA, Buttler MG, d'…

… digoxigenin-llUTP or [35S]UTP by in vitro transcription. A synthetic SVS II oligonucleotide …
of the ligand structure on immunochemical detection of the hapten. Histochemistry 84:169-178 …

… conditions for RNA oligonucleotide probes conjugated to a fluorescein hapten. We …
oligonucleotide probes in C. HuC was detected by standard in situ hybridization using an in vitro …

… phosphatase detection of the digoxigenin reporter molecule. … digoxigenin-labeled, 16S
rRNA-targeted oligonucleotides. … of RNA transcripts in vitro with the hapten digoxigenin (DIG); …

… probe labeled with the hapten digoxigenin. Hybrids are detected … to PCR 1 except that oligo
G was used as the 3’ PCR 2 primer … oligo E and unlabeled oligo F were used as the 5’ PCR 2 …

… We confirmed that the labeling reaction produced the expected amount of labeled
oligonucleotides by dot blot. Nuclear extracts were prepared as described previously [8]. Nuclear …

… oligonucleotide. Then, the activated thiol group of the oligonucleotide reacts with a maleimide
modified hapten (… labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing …