An in vitro model for mineralization of human osteoblast-like cells on implant materials

M Ahmad, MB McCarthy, G Gronowicz - Biomaterials, 1999 - Elsevier
cells produced osteocalcin while U2-Os and Saos-2 cells did not [60]. Our unpublished data
of mineralizing cultures … D treated Saos-2 cultures had a low amount of osteocalcin mRNA. …

Cell culture systems for studies of bone and tooth mineralization

AL Boskey, R Roy - Chemical reviews, 2008 - ACS Publications
… One example in which new mineral deposition (as opposed to the existing mineral in the
ceramic) was characterized used SEM and EDAX to study rat osteoblast cells cultured with 10 …

Expression of collagen, osteocalcin, and bone alkaline phosphatase in a mineralizing rat osteoblastic cell culture

P Collin, JR Nefussi, A Wetterwald, V Nicolas… - Calcified tissue …, 1992 - Springer
mineralizing rat osteoblastic cell culture system synthesizes collagen type I, V, and traces of
type III, osteocalcin, and bone ALP isoenzyme. Medium osteocalcin … the mineralization phase…

Calcification as an indicator of osteoinductive capacity of biomaterials in osteoblastic cell cultures

HA Declercq, RMH Verbeeck, LI De Ridder… - Biomaterials, 2005 - Elsevier
culture type and the observed mineralization are described. In the present paper, we
compared mineralization in cultures of UMR-106 rat osteosarcoma cells and osteoblastic cells

Temporal expression of PDGF receptors and PDGF regulatory effects on osteoblastic cells in mineralizing cultures

X Yu, S Hsieh, W Bao… - … of Physiology-Cell …, 1997 - journals.physiology.org
… This study demonstrates that, in long-term mineralizing cultures of rat calvarial osteoblastic
cells, continuous treatment of PDGF stimulates the proliferation of osteoblasts at the early …

Osteoblasts isolated from mouse calvaria initiate matrix mineralization in culture.

B Ecarot-Charrier, FH Glorieux… - The Journal of cell …, 1983 - rupress.org
… also observed in rat fibroblasts cultured under similar conditions. … osteoblasts that retain in
culture the ability to form a calcified type I collagen matrix. Mineralization occurring in cultures

Matrix mineralization and the differentiation of osteocyte‐like cells in culture

Y Mikuni‐Takagaki, Y Kakai, M Satoyoshi… - Journal of Bone and …, 1995 - academic.oup.com
… we have isolated osteoblastic and osteocytic cells. Bone sialoprotein … cells but not in
osteoblastic cells. We discuss the possibility that differentiation of young osteocytes from osteoblasts

[HTML][HTML] Rapidly Forming Apatitic Mineral in an Osteoblastic Cell Line (UMR 106—01 BSP)(∗)

CM Stanford, PA Jacobson, ED Eanes… - Journal of Biological …, 1995 - ASBMB
… that cultured UMR cells rapidly form … mineralization within the cell layer increased as a
function of increasing β-GP or P i supplementation in confluent cultures of UMR 106-01 BSP cells

Expression of differentiated function by mineralizing cultures of chicken osteoblasts

LC Gerstenfeld, SD Chipman, J Glowacki, JB Lian - Developmental Biology, 1987 - Elsevier
… total osteocalcin amount observed. In /3-GP04-treated cultures, the osteocalcin levels
associated with the cell layer were between 2 to 5 times greater than those of the control cultures. …

ATP-mediated mineralization of MC3T3-E1 osteoblast cultures

Y Nakano, WN Addison, MT Kaartinen - Bone, 2007 - Elsevier
… This dual function for TG2, acting as both a TGase and an ATPase during different phases
of matrix formation and mineralization in osteoblast cultures in vitro, is also supported by our …