[HTML][HTML] p190RhoGAP proteins contain pseudoGTPase domains
AL Stiegler, TJ Boggon - Nature communications, 2017 - nature.com
The two p190RhoGAP proteins, p190RhoGAP-A and-B, are key regulators of Rho GTPase
signaling and are essential for actin cytoskeletal structure and contractility. Here we report
the discovery of two evolutionarily conserved GTPase-like domains located in the 'middle
domain', previously thought to be unstructured. Deletion of these domains reduces RhoGAP
activity. Crystal structures, MANT-GTPγS binding, thermal denaturation, biochemical assays
and sequence homology analysis all strongly support defects in nucleotide-binding activity …
signaling and are essential for actin cytoskeletal structure and contractility. Here we report
the discovery of two evolutionarily conserved GTPase-like domains located in the 'middle
domain', previously thought to be unstructured. Deletion of these domains reduces RhoGAP
activity. Crystal structures, MANT-GTPγS binding, thermal denaturation, biochemical assays
and sequence homology analysis all strongly support defects in nucleotide-binding activity …
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