… kinetic model of PCR amplification (Equation 1) and a constant PCR efficiency. Cycle-dependent
changes in PCR efficiency are predicted by sigmoid models used in qPCR analysis (20,…

… This single-stranded binding protein has also been shown to enhance PCR amplification of
difficult templates (26). In addition, retro-viral capsid proteins have been shown to bind single…

… In general, the variability of results observed in repeated quantitative analyses of individual
STR-PCR specimens is in a range under 5%. However, it is advisable to analyze the last …

… PCR as being incapable of quantitative analysis. Initially, PCR was not viewed as having
great quantitative … factor that makes PCR so powerful, ie, its ability to amplify small amounts of …

… input amounts of nucleic acid and that each sample under analysis amplifies with identical
efficiency up to the point of quantitative analysis. A gene sequence (contained in all samples …

… the simultaneous measurement of final products of PCR amplification. For this purpose several
approaches have … Quantitative analysis of specific mRNA transcripts using a competitive …

… amplification in the aphid Myzus persicae [31]. The LightCycler offers a reproducibly accurate
measurement of amplification … other PCR quantification methods that use endpoint analysis…

… Our results indicate that simple methods that do not rely on the estimation of the efficiency
of the PCR amplification may provide reproducible and sensitive data, but that they do not …

… analysis mode, in which the level of amplified DNA is continuously monitored over the course
of amplification, can provide this quantitative … normalization and quantitative analysis. To …

… The assay has a very large dynamic range of target molecule determination because the
real-time measurement of the PCR product enables us to quantify the amplified products in the …