Detection of Staphylococcus aureus Enterotoxins A to D by Real-Time Fluorescence PCR Assay

…, S Opper, K Heeg, S Zimmermann - Journal of Clinical …, 2003 - Am Soc Microbiol
PCR assay for the detection of SE genes, we focused on the four classically described
enterotoxins of S. … PCR assay (TaqMan PCR) that detects not only the genes for SEA to SED of …

PCR‐based procedures for detection and quantification of Staphylococcus aureus and their application in food

B Alarcon, B Vicedo, R Aznar - Journal of applied microbiology, 2006 - academic.oup.com
PCR detection of Staphylococcus aureus in different food matrices and to establish a RTQ‐PCR
On the basis of these results, the SYBR‐Green I‐based RTQ‐PCR assay developed in …

Development and evaluation of a PCR-based immunoassay for the rapid detection of methicillin-resistant Staphylococcus aureus

KJ Towner, DCS Talbot, R Curran… - Journal of medical …, 1998 - microbiologyresearch.org
… -immunoassay described in the present paper offers a particularly user-friendly format and,
overall, the PCR-immuno-assay technique seemed to be more accurate than conventional …

Comparison of PCR-Based Methods for Typing Staphylococcus aureus Isolates

A Sabat, N Malachowa, J Miedzobrodzki… - Journal of clinical …, 2006 - Am Soc Microbiol
… [coa, spa, and HVR], and STAR-RP) have been poorly evaluated in comparison to the
well-established assays (RAPD PCR and rep-PCR). The new methods require careful …

Comparison of a polymerase chain reaction assay and a conventional microbiologic method for detection of methicillin-resistant Staphylococcus aureus

Y Tokue, S Shoji, K Satoh, A Watanabe… - Antimicrobial Agents …, 1992 - Am Soc Microbiol
… by the polymerase chain reaction (PCR) and Southern blot analyses. The results were
analyzed in relation to those of the MIC assay of methicillin and oxacillin. PCR assay results were …

Development of a Single-Reaction Multiplex PCR Toxin Typing Assay for Staphylococcus aureusStrains

NK Sharma, CED Rees, CER Dodd - Applied and Environmental …, 2000 - Am Soc Microbiol
… it is possible, using a single PCR assay, to rapidly screen staphylococcal isolates for the
presence of the different enterotoxin genes which are routinely detected by serological test. The …

New Real-Time PCR Assay for Rapid Detection of Methicillin- Resistant Staphylococcus aureus Directly from Specimens Containing a Mixture of Staphylococci

A Huletsky, R Giroux, V Rossbach… - Journal of clinical …, 2004 - Am Soc Microbiol
aureus, either of which can carry mecA. In this study, we describe a real-time multiplex PCR
assay … containing a mixture of staphylococci in <1 h. Five primers specific to the different …

Multiplex PCR Assay for Simultaneous Detection of Nine Clinically Relevant Antibiotic Resistance Genes in Staphylococcus aureus

B Strommenger, C Kettlitz, G Werner… - Journal of clinical …, 2003 - Am Soc Microbiol
PCR assay for the detection of nine clinically relevant antibiotic resistance genes of
Staphylococcus aureus. … A resistance) simultaneously in one PCR amplification. An additional …

… between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of Staphylococcus aureus and Staphylococcus …

F Martineau, FJ Picard, N Lansac… - Antimicrobial agents …, 2000 - Am Soc Microbiol
aureus- and S. epidermidis-specific PCR assays used in this study have been previously
described by … The various multiplex PCR assays developed in this study are given in Table 2. A …

A pentaplex PCR assay for the rapid detection of methicillin-resistant Staphylococcus aureus and Panton-Valentine Leucocidin

H Al-Talib, CY Yean, A Al-Khateeb, H Hassan… - BMC microbiology, 2009 - Springer
… The pentaplex PCR assay developed was rapid and gave results within 4 h, which is … of
Staphylococcus spp., virulence and their resistance to methicillin. Our PCR assay may be used …