… within the assay will bind and generate an MRSA-specific amplicon during the PCR, which
is … We describe the validation of this assay for the detection of MRSA from swabs obtained …

… PCR showed a sensitivity … the different PCR assays resulting from this study were lower
than those previously described. These findings could be the result of the nature of the specimen …

… Accordingly, in this study, we mainly aimed to evaluate specimen types and specimen …
RT-PCR testing. We hope to assist other laboratories in selecting appropriate specimen for the …

… patients (23 specimens) had one or more specimens that were inhibited on the initial PCR
assay but that were not inhibited on the assay of the stored frozen lysate specimens. These 18 …

… of a real-time PCR assay with the LightCycler system for the … -time PCR assay directly with
rectal swabs and enrichment … -time PCR was evaluated with clinical surveillance specimens …

… PCR: one by the TMA assay for the vaginal specimen, one by the PCR assay for the cervical
swab specimen, and one by the PCR assay for the urine specimen (these are indicated in …

… real-time PCR assay were tested in a confirmatory PCR which … the 524 specimens tested
by both culture and real-time PCR, … -time PCR assay; 16 of the 18 discrepant specimens were …

… site that would have reduced the sensitivity of the assay. The sensitivities of the RT-PCR
assays directly on nasal swab specimens depend on several other factors, including the viral …

… -time HSV PCR assays evaluated in this study clearly have a role as highly sensitive and
specific assays for the enhanced detection of HSV from dermal, ocular and genital swabs. The …

… amplification curves between adult and neonate specimens. An example of a real-time PCR
assay run is in Figure 1. A total of 299 paired nasal swabs were collected from neonates in …