Somatic embryogenesis and plantlet development in Pinus sylvestris and Pinus pinaster on medium with and without growth regulators

MA Lelu, C Bastien, A Drugeault… - Physiologia …, 1999 - Wiley Online Library
… Germination of cotyledonary somatic embryos and plantlet development from different
embryogenic lines of Pinus syl6estris (G−) maintained on proliferation medium without growth

Improving plantlet yield in Pinus pinaster somatic embryogenesis

JM Alvarez, N Bueno, M Cortizo… - Scandinavian Journal of …, 2013 - Taylor & Francis
… embryos able to develop properly into well-growing plants. In this work, we present an
improved protocol focused on increasing the maturation and conversion rate of Pinus pinaster Ait. …

Adventitious shoot formation and plant regeneration from Pinus pinaster Sol. ex Aiton

F Calixto, MS Pais - In Vitro Cellular & Developmental Biology-Plant, 1997 - Springer
Pinus pinaster (Maritime pine) is one of the three most important pine species in Europe (16)…
this paper, plantlet height doubled during rooting. In addition, removal of plantlets was easy, …

In vitro regeneration of Pinus pinaster adult trees

N De Diego, IA Montalbán… - Canadian journal of …, 2008 - cdnsciencepub.com
… In this study, shoot buds were collected from 20 maritime pine (Pinus pinaster Ait.) adult …
/L 6-benzyladenine developed healthy and well rooted plantlets. The ability to micropropagate …

In vitro adventitious budding on Pinus pinaster cotyledons and needles

A David, H David, T Mateille - Physiologia plantarum, 1982 - Wiley Online Library
… of Pinus pinaster explants, which are different from one another in terms of anatotnical structure
and physiological development: … Reliable plantlet fonnation from embryos and seedling …

Simplified and improved somatic embryogenesis for clonal propagation of Pinus pinaster (Ait.)

MA Lelu-Walter, M Bernier-Cardou, K Klimaszewska - Plant Cell Reports, 2006 - Springer
… In this study, several improvements and simplifications of SE protocols in Pinus pinaster (Ait.),
a … The in vitro-developed plantlets were characterized by a vigorous growth of shoots and …

Somatic Embryogenesis in Maritime Pine (Pinus Pinaster)

J Bercetche, M Pâques - … Embryogenesis in Woody Plants: Volume 3 …, 1995 - Springer
… somatic embryo development. We have characterised the cell lines initiated in Pinus pinaster
into … Plantlet development from immature zygotic embryos of hybrid larch through somatic …

Somatic Embryogenesis in Maritime Pine (Pinus pinaster Ait.)

L Harvengt - Protocol for somatic embryogenesis in woody plants, 2005 - Springer
… After one month, the plastic sheet is progressively removed and the plantlets treated as
standard seedlings. Fortunately, plantlets lacking primary root development are usually able to …

Zygotic and somatic embryo morphogenesis in Pinus pinaster: comparative histological and histochemical study

S Tereso, K Zoglauer, A Milhinhos, C Miguel… - Tree …, 2007 - academic.oup.com
… of storage proteins and starch in Pinus pinaster Ait. zygotic embryos with those in somatic …
plantlet development in Pinus sylvestris and Pinus pinaster on medium with or without growth

An improved micropropagation protocol for maritime pine (Pinus pinaster Ait.) isolated cotyledons

JM Alvarez, J Majada, RJ Ordás - Forestry, 2009 - academic.oup.com
… Shoot development and proliferation was obtained by transfer to hormone-free medium with
… shoot development. Based on 86 per cent rooting success rate, at least 70 plantlets can be …