… with specific primers in a second nested PCR. The amplification strategy used for genotyping
P. falciparum parasites is the nested PCR. In the first amplification reaction oligonucleotide …

… the results of PCR-based assays of high frequency SNPs (data not shown). Genotyping data
from these … We expect the genotyping data from mixed samples to predominantly reflect the …

… blood samples from 104 asymptomatic persons from a malaria-endemic village in Northern
Nigeria aged between 5 and 70 years by PCR-RFLP for the msp2 genotypes of their P. …

… The complexity of infection and the frequency of multiple infections were calculated by
combining the msp1 and msp2 PCR genotyping results. The highest number of bands detected, …

… Reinfections were defined as the absence of matching bands either after pfmsp2 or pfmsp1
genotyping. A negative PCR result on either pfmsp2 or pfmsp1 analysis was considered as …

… the genotyping of the major polymorphisms of pfmdr1 and pfcrt genes using rapid-cycle PCR
and … drug resistance of large number of samples at the phenotypic and genotypic levels. …

… genotyping assays using high-resolution melting (HRM) analysis to detect molecular biomarkers
associated with drug resistance across six genes in Plasmodium falciparum. … PCR using …

… genetically distinct Plasmodium falciparum parasite populations. … densities and circulating
genotypes in asymptomatic Senegalese … falciparum, both by microscopy and PCR, while other …

… Studying drug resistance in Plasmodium falciparum requires … infections; infections with
single genotype or having a minor … mixtures correlated with genotypes using polymerase chain …

… Here, using PCR at the msp1 and 2 loci, we explore the relationship between the traditional
… falciparum entails MOI estimation using genotype data from the msp-1 and -2 loci [20]. This …