Rapid and sensitive detection of porcine torovirus by a reverse transcription loop-mediated isothermal amplification assay (RT-LAMP)

X Liu, Y Zhou, F Yang, P Liu, Y Cai, J Huang… - Journal of virological …, 2016 - Elsevier
Porcine torovirus (PToV) is associated with swine gastroenteritis, but its pathogenesis is …
-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the …

Identification and characterization of a porcine torovirus

A Kroneman, L Cornelissen, MC Horzinek… - Journal of …, 1998 - Am Soc Microbiol
Torovirus RNA was detected by use of an RT PCR assay targeted to the NTR of the viral
genome with oligonucleotides 293 and 294 as primers. For RT PCR amplification of the PoTV N-…

[HTML][HTML] Porcine Torovirus (PToV)—a brief review of etiology, diagnostic assays and current epidemiology

ZM Hu, YL Yang, LD Xu, B Wang, P Qin… - Frontiers in Veterinary …, 2019 - frontiersin.org
Porcine torovirus (PToV) is a potential enteric swine … co-infections with other swine pathogens
and intertype recombination … , relationship with other Toroviruses, virion morphological …

Detection of porcine torovirus by real time RT-PCR in piglets from a Spanish farm

J Pignatelli, M Jiménez, L Grau-Roma… - Journal of virological …, 2010 - Elsevier
… As expected, the nucleotide sequences of all the RT-PCR amplified fragments were shown
to be related to torovirus N gene sequences, thus confirming the specificity of the method. …

Detection and molecular characterization of porcine toroviruses in Korea

DJ Shin, SI Park, YJ Jeong, M Hosmillo, HH Kim… - Archives of …, 2010 - Springer
This study examined the prevalence and genetic diversity of the porcine torovirus (PToV) in
Korea. Of 295 samples, 19 (6.4%) samples tested positive for PToVs by RT-PCR. A low …

Development of universal SYBR Green real-time RT-PCR for the rapid detection and quantitation of bovine and porcine toroviruses

MDT Hosmillo, YJ Jeong, HJ Kim, TM Collantes… - Journal of Virological …, 2010 - Elsevier
… used in this study likely resulted in more efficient amplification and higher analytical sensitivity.
Despite the shorter amplicon size in PToV nested PCR, the real-time PCR reproduced …

A novel spike subunit 1‐based enzyme‐linked immunosorbent assay reveals widespread porcine torovirus infection in eastern China

P Qin, YL Yang, ZM Hu, YQ Zhang… - Transboundary and …, 2022 - Wiley Online Library
… -positive faecal sample by RT-PCR amplification of overlapping regions spanning the entire
genome. Viral cDNAs were amplified by SuperScript II with random primers according to the …

Development of a TaqMan-probe-based multiplex real-time PCR for the simultaneous detection of emerging and reemerging swine coronaviruses

Z Pan, J Lu, N Wang, WT He, L Zhang, W Zhao, S Su - Virulence, 2020 - Taylor & Francis
amplification curves represent samples positive for PEDV, PDCoV, PToV, and SADS-CoV
detected by our multiplex real-time PCR assay… of coronavirus and torovirus, we cannot …

Molecular epidemiology of porcine torovirus (PToV) in Sichuan Province, China: 2011–2013

L Zhou, H Wei, Y Zhou, Z Xu, L Zhu, J Horne - Virology journal, 2014 - Springer
… diarrhea (equine torovirus, EToV, also called Berne virus) in 1972 [8]. To date, research on
toroviruses has been limited because of difficulties associated with their propagation in vitro. …

Longitudinal serological and virological study on porcine torovirus (PToV) in piglets from Spanish farms

J Pignatelli, L Grau-Roma, M Jimenez, J Segales… - Veterinary …, 2010 - Elsevier
… A study was performed to evaluate porcine torovirus (PToV) seroprevalence and infection in
… To amplify the complete HE gene from rectal swab samples, an amplification reaction was …