Isolation of Porcine reproductive and respiratory syndrome virus (PRRSV) from oral fluids
was first reported in 1997. The objective of the present study was to determine whether …

… porcine torovirus in GenBank. In the specificity test, we used this method to amplify pseudorabies
virus, porcine … indicated that only porcine torovirus was successfully amplified a target …

… and amplification in susceptible cells. In addition, the USDA regulations … testing of porcine
trypsin for porcine parvovirus by immunofluorescence upon exposure and amplification …

… porcine reproductive and respiratory syndrome virus (PRRSV) in cell cultures and in formalin-fixed
paraffin-embedded tissue sections originating from experimentally infected pigs … pigs …

… report of toroviruses in horses and cattle (83, 90), torovirus-like (… reports highlighting the
presence of toroviruses in humans (4–7… such as horses, sheep, goats, and pigs (33–35, 84, 86). …

… For an example, a new porcine coronavirus, has emerged through recombination between
… the multi-target amplification tests which currently require multiple testing modalities. These …

… quantitative PCR, each system testing three targets, to ensure simultaneously amplification
and fluorescent signal acquisition, effectively testing six kinds of viruses simultaneously. …

… that both PBV strains were highly similar (99.6% nucleotide sequence identity) and clustered
closer to the related human PBV genotype II reference strains than those of porcine PBV …

… for simultaneous amplification of all three virus-specific genes, such as denaturation,
annealing, elongation and final elongation temperature and the required number of amplification …

… In pigs, CoVs and toroviruses (ToVs) affect a variety of organs, including the gastrointestinal
and respiratory tracts, the peripheral and central nervous systems (CNS), and the mammary …