Base editing: precision chemistry on the genome and transcriptome of living cells
… The ability to precisely and efficiently edit DNA sequences within the genome of living cells
… (DSB) at a precise target location in the genome of a wide variety of cells and organisms 5,6,7…
… (DSB) at a precise target location in the genome of a wide variety of cells and organisms 5,6,7…
Chemically engineering cells for precision medicine
Y Wang, Z Li, F Mo, TJ Chen-Mayfield, A Saini… - Chemical Society …, 2023 - pubs.rsc.org
… A bunch of reaction types, such as acyl imidazole chemistry and tosyl chemistry, are proved
feasible for labeling living cells using this approach. For instance, Reddi et al. utilized the …
feasible for labeling living cells using this approach. For instance, Reddi et al. utilized the …
[HTML][HTML] Real-time precision opto-control of chemical processes in live cells
MG Clark, GA Gonzalez, Y Luo… - Nature …, 2022 - nature.com
… precision control of molecular states of a photochromic molecule in different regions of the
cells. We … in live cells. RPOC can automatically detect and control biomolecular activities and …
cells. We … in live cells. RPOC can automatically detect and control biomolecular activities and …
Light-activated tetrazines enable precision live-cell bioorthogonal chemistry
L Liu, D Zhang, M Johnson, NK Devaraj - Nature chemistry, 2022 - nature.com
… the presence of living cells. … cells in a population 19 . Similarly, light-controllable tetrazine
ligations would open up applications such as cell surface engineering with single-cell precision …
ligations would open up applications such as cell surface engineering with single-cell precision …
Experimental approaches for addressing fundamental biological questions in living, functioning cells with single molecule precision
… Fluorescence microscopy can generate exceptional measurement contrast for the detection
of tiny signals emitted from single molecule fluorescent tags in the milieu of a living cell, …
of tiny signals emitted from single molecule fluorescent tags in the milieu of a living cell, …
Organelle tracking in a living cell with microsecond time resolution and nanometer spatial precision
… microscopy,11 all with 1–2 nm spatial precision. However, the best time resolution in these
… nm spatial precision in live cells. This high time-resolution and spatial precision are made …
… nm spatial precision in live cells. This high time-resolution and spatial precision are made …
[HTML][HTML] Live-cell labeling of endogenous proteins with nanometer precision by transduced nanobodies
A Klein, S Hank, A Raulf, EF Joest, F Tissen… - Chemical …, 2018 - pubs.rsc.org
… knowledge the first labeling of an endogenous protein inside living cells by exogenously
derived fNbs. By contrast, in live HeLa Kyoto cells expressing α-Lamin-EGFP chromobodies, …
derived fNbs. By contrast, in live HeLa Kyoto cells expressing α-Lamin-EGFP chromobodies, …
Nanosensor chemical cytometry: advances and opportunities in cellular therapy and precision medicine
Y Song, C Tian, Y Lee, M Yoon, SE Yoon… - ACS Measurement …, 2023 - ACS Publications
… (68,69) NCC utilizes the inherent optical lensing effect of live cells in a cell guiding channel
to extract single cell information in a completely label-free way and associate biomolecular …
to extract single cell information in a completely label-free way and associate biomolecular …
Living Cell‐Derived Intelligent Nanobots for Precision Oncotherapy
L Zhou, K Li, Y Liu, R Zhang, Y Yao… - Advanced Functional …, 2024 - Wiley Online Library
… cell biological nanobot systems in precision oncology, including: i) engineered artificial cells
as “… anti-tumor therapeutic strategies of engineered living cell nanorobots under external …
as “… anti-tumor therapeutic strategies of engineered living cell nanorobots under external …
DNA-templated bioorthogonal reactions via catalytic hairpin assembly for precise RNA imaging in live cells
SJ Zhao, P Zheng, Z Wu, JH Jiang - Analytical Chemistry, 2022 - ACS Publications
… decaging of vinyl ether for precise mRNA imaging in live cells, as illustrated in Scheme 1. …
in live cells and further distinguish the mRNA expression level of tumor cells to normal cells. …
in live cells and further distinguish the mRNA expression level of tumor cells to normal cells. …
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