Statistical design of experiments for protein crystal growth and the use of a precrystallization assay

CW Carter Jr, ET Baldwin, L Frick - Journal of Crystal Growth, 1988 - Elsevier
… signs for protein crystallization … of protein crystal growers, evidencedby therapidly ing
between 20 and 35 experiments to screen conditions for growing crystals of six new proteins

Monitoring crystallization experiments using dynamic light scattering: Assaying and monitoring protein crystallization in solution

WW Wilson - Methods, 1990 - Elsevier
… Another important advance in protein crystal growth technology is the statistical design of …
Several precrystallization assay methods have been proposed or utilized for this purpose and …

Development of an automated high throughput LCP-FRAP assay to guide membrane protein crystallization in lipid mesophases

F Xu, W Liu, MA Hanson, RC Stevens… - Crystal growth & …, 2011 - ACS Publications
precrystallization assay for membrane proteins, which is based on screening diffusion properties
of proteins in … The assay allows selecting the most promising protein construct, ligands, …

Small-scale approach for precrystallization screening in GPCR X-ray crystallography

M Audet, K Villers, J Velasquez, M Chu, C Hanson… - Nature protocols, 2020 - nature.com
… to precrystallization screening 11 and allows the simultaneous processing of many sets of
conditions (we test … receptor modifications that could lead to crystal growth and diffraction is not …

A historical perspective on protein crystallization from 1840 to the present day

R Giegé - The FEBS journal, 2013 - Wiley Online Library
… the physical chemistry of protein crystal growth and the use of … also how parameters vary in
a crystallization assay (from the … phase constituted by precrystalline protein clusters displays …

[6] Second virial coefficient as predictor in protein crystal growth

A George, Y Chiang, B Guo, A Arabshahi, Z Cai… - Methods in …, 1997 - Elsevier
… that present unusual difficulties it may be critical to incorporate a reliable, quantitative
precrystallization assay that can predict the outcome of crystal growth experiments. …

Blocking rapid ice crystal growth through nonbasal plane adsorption of antifreeze proteins

LLC Olijve, K Meister, AL DeVries… - Proceedings of the …, 2016 - National Acad Sciences
… to growing ice crystal surfaces and arrest further ice growth. … (TH) and ice recrystallization
inhibition (IRI) activity of all … , sonocrystallization, and recrystallization assays. The results show …

Dynamics of layer growth in protein crystallization

PG Vekilov, JID Alexander - Chemical reviews, 2000 - ACS Publications
… when purified by recrystallization, showed no difference in crystal growth rate and … to test
the above rationale by analyzing its predictions for the results of actual protein crystal growth

A fluorescence-detection size-exclusion chromatography-based thermostability assay for membrane protein precrystallization screening

M Hattori, RE Hibbs, E Gouaux - Structure, 2012 - cell.com
Growth of membrane protein crystals for the purpose of determining the underlying atomic
… the protein of interest. This “precrystallization” screening is usually performed with purified …

Dynamics of protein precrystallization cluster formation

Y Georgalis, A Zouni, W Saenger - Journal of crystal growth, 1992 - Elsevier
… of biological macroIn recent years there has been a growing inter- … on lysozyme crystal est
in developing precrystallization assays. … growth, employing powerspectroscopy, were pubidea …