A universal real-time PCR assay for the quantification of group-M HIV-1 proviral load

MS Malnati, G Scarlatti, F Gatto, F Salvatori… - Nature protocols, 2008 - nature.com
… design of a universal assay 34,35,36 . We have developed an HIV-1 group M-specific
quantitative real-time PCR assay that measures the HIV-1 proviral DNA load with a similar degree …

Quantitation of HTLV-I proviral load by a TaqMan real-time PCR assay

A Dehée, R Césaire, N Désiré, A Lézin… - Journal of virological …, 2002 - Elsevier
PCR assay was developed to quantify HTLV-I DNA using a target sequence located in the
pol gene of the virus. The HTLV-I proviral load … the reproducibility of the assay to be improved. …

A quantitative PCR method for the assay of HIV-1 provirus load in peripheral blood mononuclear cells

JM Bennett, S Kaye, N Berry, RS Tedder - Journal of virological methods, 1999 - Elsevier
… The measurement of provirus DNA load in peripheral blood … A quantitative PCR assay was
developed for HIV-1 provirus … at the low copy numbers of provirus seen in clinical samples. …

Quantification of human immunodeficiency virus type 1 proviral load by a TaqMan real-time PCR assay

N Désiré, A Dehée, V Schneider… - Journal of clinical …, 2001 - Am Soc Microbiol
proviral HIV-1 DNA using real-time PCR that would be sensitive and reproducible and have
a high throughput. Proviral … that HAART decreases the proviral load but has also shown that …

Quantification of proviral load of human immunodeficiency virus type 2 subtypes A and B using real-time PCR

F Damond, D Descamps, I Farfara… - Journal of clinical …, 2001 - Am Soc Microbiol
… We evaluated a real-time PCR assay with the LightCycler system for HIV-2 proviral DNA
quantitation in clinical specimens. The LightCycler system is simple and rapid and offers a …

Quantitation of feline leukaemia virus viral and proviral loads by TaqMan® real-time polymerase chain reaction

R Tandon, V Cattori, MA Gomes-Keller, ML Meli… - Journal of virological …, 2005 - Elsevier
… ® PCR assay to quantify plasma FeLV RNA loads during … For appropriate quantitation of
total FeLV loads and reliable … time PCR assay for the determination of FeLV proviral loads. …

Quantitation of HTLV-I and II proviral load using real-time quantitative PCR with SYBR Green chemistry

TH Lee, DM Chafets, MP Busch, EL Murphy - Journal of clinical virology, 2004 - Elsevier
… Green quantitative PCR assay to measure the proviral load of HTLV-I and HTLV-II in
peripheral blood mononuclear cells (PBMCs). We optimized the conditions of the assay including …

Validation of a quantitative real-time PCR assay for HTLV-1 proviral load in peripheral blood mononuclear cells

C Rosadas, MJ Cabral-Castro, ACP Vicente… - Journal of virological …, 2013 - Elsevier
… The objective of this study was to validate a TaqMan real-time PCR assay for HTLV-1 proviral
load detection in peripheral blood mononuclear cells. TARL-2 cells were used to generate …

Development and validation of a multiplex real-time PCR assay for simultaneous genotyping and human T-lymphotropic virus type 1, 2, and 3 proviral load …

B Moens, G López, V Adaui, E González… - Journal of clinical …, 2009 - Am Soc Microbiol
proviral load determination of our multiplex assay was compared with that of a previously
published HTLV-1 singleplex quantitative PCR … 0.87) correlation between proviral load values …

Development and validation of a real-time PCR assay for a novel HTLV-1 tax sequence detection and proviral load quantitation

GM Castro, MC Balangero, E Maturano… - Journal of virological …, 2013 - Elsevier
… using SYBR Green dye was established in order to detect and quantify the proviral DNA of
… the assay was standardized to amplify a novel, conserved HTLV-1 tax region. Proviral load