… tool based on real-time polymerase chain reaction was developed to detect the three
pathogenic fungi in a single reaction on fruit, twigs, and flowers of Prunus and Malus spp. Species-…

… and previously determined as phytoplasma free with other diagnostics protocols were used
as negative controls in the conventional PCR assays as well as in the real-time PCR assays. …

… ) Malus-, Prunus-, and Pyrus-affecting MLOs were obtained. Restriction enzyme analysis
of … restriction fragment length polymorphisms between Malus-, Prunus, and Pyrus-affecting …

… The PCR products were separated on 2% agarose gel in 1×TAE buffer. Specific PCR …
These purified PCR products were then ligated with a Peasy-T1 vector (TransGen, Beijing, …

… quantitative polymerase chain reaction (RT-qPCR… assays thus developed detected a greater
range of viroid isolates and with greater sensitivity than the current endpoint RT-PCR assays…

… na občutljivost PCR. Za … PCR v primeru, ko smo spranim celicam dodali mešanico raztopin
NaOH in SDS ter vzorec 15 minut segrevali v vodni kopeli pri 95 o C. Prag občutljivosti PCR …

… and the other half was used for PCR analysis. If strong grafts were PCR-negative, the
phytoplasma-infection of the graft tip was confirmed by PCR. As control, micrografts with healthy …

… of Prunus persica (peach) and the internal transcribed spacer region of Malus domestica (…
For our apple detection method, we chose Malus domestica as the target, because it is the …

… RFLP analysis of nested PCR products was performed using … ., 1998) and used as template
in a nested PCR assay to … Almost two thirds of the samples produced the expected PCR …

… from Prunus and Malus … test was designed as a combined RTPCR assay with primers
PDO-F1i, PDO-R3i, and PDO-R4i, from which an aliquot was used in a second nested PCR assay …