Efflux Pumps Involved in Toluene Tolerance in Pseudomonas putida DOT-T1E

JL Ramos, E Duque, P Godoy, A Segura - Journal of bacteriology, 1998 - Am Soc Microbiol
… composition explained why a proportion of the P. putida DOT-T1E cells pregrown with
toluene in the gas phase were tolerant to the solvent shock, we tested the survival of P. …

[HTML][HTML] Analysis of solvent tolerance in Pseudomonas putida DOT-T1E based on its genome sequence and a collection of mutants

…, M Fernández, L Molina, J de la Torre, P Bernal… - Febs Letters, 2012 - Elsevier
… The subject of this study is the DOT-T1E (T1E) P. putida strain that was isolated from a …
Strain T1E has been also used for the development of biotechnological biotransformation …

Three Efflux Pumps Are Required To Provide Efficient Tolerance to Toluene in Pseudomonas putidaDOT-T1E

A Rojas, E Duque, G Mosqueda, G Golden… - Journal of …, 2001 - Am Soc Microbiol
… a third inducible pump in solvent tolerance in P. putida DOT-T1E, we tested whether the
srpABC genes or their homologues were present inP. putida DOT-T1E. To this end we designed …

Involvement of the cis/trans Isomerase Cti in Solvent Resistance of Pseudomonas putidaDOT-T1E

F Junker, JL Ramos - Journal of Bacteriology, 1999 - Am Soc Microbiol
Pseudomonas putida DOT-T1E is a solvent-resistant strain that is able to grow in the presence
of high concentrations of toluene. We have cloned and sequenced the cti gene of this …

A Set of Genes Encoding a Second Toluene Efflux System in Pseudomonas putida DOT-T1E Is Linked to the tod Genes for Toluene Metabolism

G Mosqueda, JL Ramos - Journal of Bacteriology, 2000 - Am Soc Microbiol
putida DOT-T1E was grown at 30C in Luria-Bertani (LB) medium or minimal medium M9 (1) …
Mutant strains of P. putida DOT-T1E generated previously and those constructed in this …

The pGRT1 plasmid of Pseudomonas putida DOTT1E encodes functions relevant for survival under harsh conditions in the environment

L Molina, E Duque, MJ Gómez, T Krell… - Environmental …, 2011 - Wiley Online Library
Pseudomonas putida DOTT1E has the capacity to grow in the presence of high
concentrations of toluene. This ability is mainly conferred by an efflux pump encoded in a self‐…

The Ssr protein (T1E_1405) from Pseudomonas putida DOTT1E enables oligonucleotide‐based recombineering in platform strain P. putida EM42

T Aparicio, SI Jensen, AT Nielsen… - Biotechnology …, 2016 - Wiley Online Library
… the open reading frame T1E_1405 from Pseudomonas putida DOT-T1E, a plausible functional
… A test based on the phenotypes of pyrF mutants of P. putida (the yeast's URA3 ortholog) …

Biotransformation in Double-Phase Systems: Physiological Responses of Pseudomonas putida DOT-T1E to a Double Phase Made of Aliphatic Alcohols and …

A Rojas, E Duque, A Schmid, A Hurtado… - Applied and …, 2004 - Am Soc Microbiol
Pseudomonas putida strain DOT-T1E is highly tolerant to organic solvents with a logP ow of
… The aim of this study was to investigate the tolerance and growth of P. putida DOT-T1E in a …

Functional analysis of new transporters involved in stress tolerance in Pseudomonas putida DOTT1E

V García, P Godoy, C Daniels, A Hurtado… - Environmental …, 2010 - Wiley Online Library
Pseudomonas putida DOT-T1E-PS128 was only affected by the presence of bipyridyl (lag
phase [lag] = 10 h). Pseudomonas putida DOT-T1E-… Pseudomonas putida DOT-T1E-PS168 …

Physiological Characterization ofPseudomonas putida DOT-T1E Tolerance top-Hydroxybenzoate

MI Ramos-González, P Godoy… - Applied and …, 2001 - Am Soc Microbiol
Pseudomonas putida DOT-T1E was isolated as a toluene-tolerant strain. We show that it is
also able to grow on high concentrations (up to 17 g/liter [123 mM]) ofp-hydroxybenzoate (…