Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression

E Martínez-García, PI Nikel, T Aparicio… - Microbial cell …, 2014 - Springer
putida KT2440 was inspected to identify regions conspicuously liable of limiting … putida
KT2440 for the sake of upgrading its performance as a host for heterologous gene expression. …

Enhanced production of polyhydroxyalkanoates in Pseudomonas putida KT2440 by a combination of genome streamlining and promoter engineering

H Liu, Y Chen, Y Zhang, W Zhao, H Guo… - International Journal of …, 2022 - Elsevier
putida KTU-U13, a 13 GIs- and upp-deleted mutant derived from the parental strain P. putida
KT2440, was used … The red fonts indicate the modified genes. Abbreviations: gcd, glucose …

Development of a high efficiency integration system and promoter library for rapid modification of Pseudomonas putida KT2440

JR Elmore, A Furches, GN Wolff, K Gorday… - Metabolic engineering …, 2017 - Elsevier
putida is the availability of tools for sophisticated genetic engineering. The primary tools
currently used for directed genetic … 3C), improving transformation efficiency of pPolyAttP to that of …

Bio-upgrading of ethanol to fatty acid ethyl esters by metabolic engineering of Pseudomonas putida KT2440

A Sarwar, LT Nguyen, EY Lee - Bioresource Technology, 2022 - Elsevier
upgrading of ethanol to FAEEs by using a genetically engineered strain of P. putida KT2440.
putida KT2440 for the sustainable production of biodiesel as an alternative to fossil fuels in …

Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440

JI Jiménez, B Miñambres, JL García… - Environmental …, 2002 - Wiley Online Library
putida KT2440 will facilitate the rational manipulation of this strain for improvingputida
KT2440 genome, the pca genes are organized in three different clusters at positions …

The revisited genome of Pseudomonas putida KT2440 enlightens its value as a robust metabolic chassis

E Belda, RGA Van Heck… - Environmental …, 2016 - Wiley Online Library
putida KT2440 lacks the ostAB genes encoding enzymes involved in the two-step trehalose
putida KT2440 genome to a complete upgrade of its sequence annotations as means to …

Deletion of genomic islands in the Pseudomonas putida KT2440 genome can create an optimal chassis for synthetic biology applications

P Liang, Y Zhang, B Xu, Y Zhao, X Liu, W Gao… - Microbial Cell …, 2020 - Springer
… that the deletion of the restriction-modification system related gene hsdS may be crucial for
significantly improving the chromosomal integration efficiency of heterologous pathways in P. …

Adaptive laboratory evolution of Pseudomonas putida KT2440 improves p-coumaric and ferulic acid catabolism and tolerance

ET Mohamed, AZ Werner, D Salvachúa… - Metabolic engineering …, 2020 - Elsevier
… rate at the expense of hydroxycinnamic acids and presents new targets for improving P. …
and the genes identified in this work are promising targets for future improvement of P. putida

A promoter engineering-based strategy enhances polyhydroxyalkanoate production in Pseudomonas putida KT2440

Y Zhang, H Liu, Y Liu, K Huo, S Wang, R Liu… - International Journal of …, 2021 - Elsevier
… promoters for improving the PHA yield and biomass of P. … putida KT2440 can accumulate
a higher proportion of PHA within the cells [38]. In this work, we further deleted the gcd gene in …

Reconstructing ethanol oxidation pathway in Pseudomonas putida KT2440 for bio-upgrading of ethanol to biodegradable polyhydroxybutanoates

LT Nguyen, DHA Mai, A Sarwar, EY Lee - International Journal of …, 2022 - Elsevier
… Using ethanol as the sole carbon source, pAceA and pTac showed high potential for
heterologous gene expression in P. putida KT2440, therefore these promoters were used further in …