… In this study, influenza viral loads were quantified with quantitative reverse transcription-polymerase
chain reaction (qRT-PCR) using viral … qRT-PCR detected as few as 10 3 …

… influenza viruses, we found that 18S rRNA is the most stable reference gene for normalising
qRT-PCR … and GPADH) were highly affected by influenza virus infection and hence are not …

… were analysed with the novel pandemic H1N1 influenza real-time PCR assay as well as the
… with human influenza viruses, we tested cell culture supernatants of human influenza virus …

… influenza A(H1N1)v virus infections, necessary to guide public health actions. Real-time
PCR is widely considered the gold standard for molecular detection of influenza viruses due to …

… real-time PCR assay for the rapid and simultaneous detection of influenza viruses (influenza
virus A, influenza virus … We also compare this real-time PCR assay to conventional culturing …

… Tests for pan-type A influenza detection are often targeted to the conserved matrix (M) … viral
RNA to detectable levels of PCR DNA is the same for real-time PCR as it is for standard PCR…

… PCR (RT-PCR) methods for the detection of novel influenza A(… Real-time polymerase
chain reaction (PCR) is more rapid and sensitive than traditional techniques including virus …

… In order to overcome these limitations, we developed a real-time multiplex PCR assay for
influenza viruses and HRSV that uses the melting-curve-analysis feature of the LightCycler …

… for the real time qPCR assays along with stability of the assay components. Cross validation
of the real time PCR … subtypes of cultured virus and a panel of influenza positive throat swab …

… ) typing of influenza viruses using SHRT-PCR. … influenza virus A (A-MP), influenza virus B
(B-MP), and hemagglutinin (HA) of the 2009 pandemic S-OIV and H5N1 avian influenza viruses…