… quantitative real time RT-PCR specific for M gene was used to determine influenza A viral
loads … , H3N2 and 2009 pandemic H1N1 virus), we found that all NPA and NS specimens were …

… -step real time reverse transcription-PCR (RT-PCR) was developed to detect the novel H7N9
virus. … Considering the high variation frequency in sequences of influenza viruses, we didn’t …

… assays for detection of respiratory syncytial virus (RSV), influenza virus type A (FluA), … study,
real-time PCR was a significant improvement over FA for detection of respiratory viruses in …

… influenza A/H1N1/2009 virus, and those of other human and swine origin influenza viruses,
… cross talk between the different channels of the real time PCR instrument. The 20-μl reaction …

… particles per infectious virus (characterized using real-time PCR and a cell culture-based
infectivity assay, data not shown)–a ratio consistent with reports from other laboratories [36]–[38…

… with qRT-PCR approaches to detect influenza viruses in water … Inhibition of qRT-PCR reactions
will also result in a higher … and low concentration of qRTPCR inhibiting substances would …

… have low influenza virus A titers by Prodesse ProFlu+ PCR (a mean real-time PCR threshold
… ± 2.0), which included the majority of the samples called influenza virus A “equivocal” or “no …

… Quantitative determinations by real-time PCR are indirect and … influenza virus or RSV
were retrospectively identified through results of routine clinical testing by qualitative PCR (qPCR; …

… The first multiplex real-time PCR tested for influenza A virus, influenza B virus, and RSV; the
… No H5 influenza virus was detected. Five real-time PCR-positive specimens were found to …

… virus lineages called the Yamagata and Victoria lineages. We present the first real-time PCR
assay for virus … , which would greatly speed up and thus improve influenza virus surveillance…