Development and validation of direct PCR and quantitative PCR assays for the rapid, sensitive, and economical detection of porcine circovirus 3

G Franzo, M Legnardi, C Centelleghe… - Journal of …, 2018 - journals.sagepub.com
… Since the identification of species Porcine circovirus 2, the relevance of genus Circovirus
on the swine industry. A new species (Porcine circovirus 3, PCV-3) has been detected in …

[HTML][HTML] Quantitative polymerase chain reaction for Porcine circovirus-2 in swine feces in a Porcine circovirus disease-affected commercial herd and a nonaffected …

KA McIntosh, JCS Harding, S Parker… - The Canadian …, 2008 - ncbi.nlm.nih.gov
… if pigs in a Porcine circovirus disease (PCVD)-affected herd (n = 100) had shed more Porcine
circovirus-2 (PCV-2) in their feces than pigs in … The PCV-2 shedding was quantified by real-…

Development and Use of a Multiplex Real-Time Quantitative Polymerase Chain Reaction Assay for Detection and Differentiation of Porcine Circovirus-2 Genotypes …

CA Gagnon, JRE Del Castillo, N Music… - Journal of …, 2008 - journals.sagepub.com
swine population had experienced a severe increase in the incidence of Porcine circovirus-…
that was associated with the emergence of a new Porcine circovirus-2 genotype (PCV-2b), …

Development and validation of a real-time PCR for the detection and quantification of porcine circovirus type 2

AM Henriques, M Duarte, SC Barros, T Fagulha… - Virusdisease, 2018 - Springer
… This paper reports the development and validation of quantitative PCR (qPCR) for the
detection of PCV2 in swine samples, using primers and a TaqMan probe directed to a conserved …

Comparison of serum pools and oral fluid samples for detection of porcine circovirus type 2 by quantitative real-time PCR in finisher pigs

GB Nielsen, JP Nielsen, J Haugegaard, SC Leth… - Porcine Health …, 2018 - Springer
Porcine circovirus type 2 (PCV2) diagnostics in live pigs often involves pooled serum and/or
oral fluid samples for group-level determination of viral load by quantitative … the PCV2 viral …

Multiplex real-time polymerase chain reaction for the differential detection of porcine circovirus 2 and 3

HR Kim, YR Park, DR Lim, MJ Park, JY Park… - Journal of virological …, 2017 - Elsevier
… Therefore, in the present study, we developed and evaluated a rapid multiplex quantitative
real-time polymerase chain reaction (mqPCR) assay using primer sets capable of …

Quantitative Detection of Porcine Torque Teno Virus in Porcine Circovirus-2–Negative and Porcine Circovirus–Associated Disease-Affected Pigs

SS Lee, S Sunyoung, H Jung, J Shin… - Journal of veterinary …, 2010 - journals.sagepub.com
… that aggravate clinical manifestation of Porcine circovirus–associated disease (PCVAD), a
… from Porcine circovirus-2–negative pigs and PCVAD-affected pigs with real-time quantitative

A methodology for porcine circovirus 2 (PCV-2) quantification based on gold nanoparticles

CR Basso, TF Cruz, BL Silva, VA Pedrosa… - Materials, 2020 - mdpi.com
The aim of the current study is to introduce a methodology aimed at producing a biosensor
that uses gold nanoparticles (AuNPs) to detect porcine circovirus 2 (PCV-2). This biosensor …

Development of real-time PCR assay for detection of porcine circovirus-like virus P1 in domestic pigs in China

K He, L Wen, Y Wang, C Lu - BMC Veterinary Research, 2015 - Springer
… the preferred method of quantitative detection of nucleic acids … PCR to accurately and
quantitatively detect P1 as well as … I fluorescence quantitative PCR detection of porcine circovirus-…

Development of primer-probe energy transfer real-time PCR for the detection and quantification of Porcine circovirus type 2

Á Bálint, M Tenk, Z Deim, T Rasmussen… - Acta Veterinaria …, 2009 - akjournals.com
porcine respiratory disease complex (PRDC). Since circoviruses commonly occur in the pig
… important not only to detect PCV2 but also to determine the quantitative aspects of viral load. …