… Selection of the preferred experimental approach for enzyme engineering … Rational design,
semi-rational design or whole-gene … The enzyme engineering approach that may have the …

… as a result of low enzyme stability or low catalytic rates or simply because no enzyme
catalyzes the desired reaction. Rational enzyme engineering promises to allow a widespread use …

… two different approaches to enzyme engineering: random approaches or (semi)rational design.
… The ultimate goal of enzyme engineering is a true rational design, which aims at de novo …

… We discuss some aspects of rational design of stabilizing mutations. In addition, we will
illustrate the possibilities and pitfalls of rational engineering of protein kinetic stability by …

… From a practical perspective, semi-rational protein engineering can … engineering. To
highlight the rapidly growing number of successful enzyme engineering studies by semi-rational …

… This review describes recent advances in enzyme engineering focusing on rational approaches
for the creation of biocatalysts with improved activity, enantioselectivity, substrate range, …

… 2.3 Semi-rational approach In this method, enzyme engineering is done by directed evolution
combined with a rational approach. Alternatively, the promising target sites for directed …

… Based on the design principles, rational computational enzyme design is … in rational
computational enzyme design, emphasizing the effectiveness and versatility of different approaches …

… should aim for a complete description of the enzymatic process: (1) substrate … an enzymatic
mechanism (11) and, as we show here, turn this extensive knowledge into enzymatic rational …

… The present review highlights the major enzyme engineering … requirement of enzyme
regulated industrial bioprocesses. … Rational techniques of enzyme engineering have been …