Autoradiographic detection of [125I]-secondary antiserum: a sensitive light and electron microscopic labeling method compatible with peroxidase …

VM Pickel, J Chan, TA Milner - Journal of Histochemistry & …, 1986 - journals.sagepub.com
We examined whether autoradiographic localization of [125I]-antirabbit immunoglobulin
(IgG) was suitable for light and electron microscopic detection of a rabbit antiserum to the …

Immunocytochemical localization of neuronal antigens: tyrosine hydroxylase, substance P,[Met5]-enkephalin.

VM Pickel - Federation Proceedings, 1979 - europepmc.org
Neuronal antigens can be demonstrated histologically by numerous direct and indirect
immunocytochemical techniques in which a specific antibody is identified by a marker …

A light and electron microscopic procedure for sequential double antigen localization using diaminobenzidine and benzidine dihydrochloride.

AI Levey, JP Bolam, DB Rye… - … of Histochemistry & …, 1986 - journals.sagepub.com
Very few double-antigen staining methods are available that are applicable to both light and
electron microscopy. The objective of this study was to develop for localization of two neural …

A combined light and electron microscopic immunocytochemical method for the simultaneous localization of multiple tissue antigens: tyrosine hydroxylase …

Z Liposits, D Sherman, C Phelix, WK Paull - Histochemistry, 1986 - Springer
In order to study the morphological interrelationships between immunocytochemically
identified neuronal systems, a double labelling procedure—suitable for correlative light and …

Ultrastructural analysis of central serotoninergic neurons immunolabeled by silver-gold-intensified diaminobenzidine chromogen. Completion of immunocytochemistry …

Z Liposits, T Görcs, K Trombitas - Journal of Histochemistry …, 1985 - journals.sagepub.com
Serotonin immunoreactive structures of the rat central nervous system (CNS) were detected
by the recently developed silver-intensified peroxidase-antiperoxidase complex (SI-PAP) …

Immunocytochemistry with internally labeled monoclonal antibodies.

AC Cuello, JV Priestley… - Proceedings of the …, 1982 - National Acad Sciences
One of the advantages of the production of monoclonal antibodies by tissue culture methods
is that they can be internally labeled by using appropriate radioactive amino acid in the …

Interchangeable uses of autoradiographic and peroxidase markers for electron microscopic detection of neuronal pathways and transmitter-related antigens in single …

VM Pickel, TA Milner - Neuroanatomical Tract-Tracing Methods 2: Recent …, 1989 - Springer
In the past several years, there has been a rapid expansion in use of immu-nocytochemical
methods for identifying neuronal molecules. These include neurotransmitter-synthesizing …

Immunocytochemistry and neurobiology

AC Cuello, JV Priestley… - Quarterly Journal of …, 1983 - Wiley Online Library
Immunocytochemistry enables the localization of transmitter‐related antigens in tissue
sections at either the light microscopic or the electron microscopic level. In the case of …

Multiple immunolabeling in histology: a new method using thermo-inactivation of immunoglobulins.

E Kolodziejczyk, AJ Baertschi - Journal of Histochemistry & …, 1986 - journals.sagepub.com
We describe a new method for successive immunofluorescence detection of multiple
peptides in single paraffin tissue sections. Immunoglobulins were inactivated at 130 degrees …

Characterization of chemically defined neurons and their cellular relationships by combined immunocytochemistry and radioautographic localization of transmitter …

O Bosler, A Beaudet, VM Pickel - Journal of electron …, 1986 - Wiley Online Library
Radioautography and immunocytochemistry may be combined at the light and electron
microscopic levels for simultaneously localizing uptake sites for exogenous transmitter …