The effect of nonaqueous solvents on the quaternary structure of viruses: a procedure for the simultaneous concentration, purification and disruption of influenza …
AR Neurath, BA Rubin, J Sillaman, H Tint - 1971 - cabidigitallibrary.org
Allantoic fluids containing either A2/Aichi/68 or B/Mass/66 influenza viruses were absorbed
onto columns of calcium phosphate. Approximately 98% of non-viral protein was removed …
onto columns of calcium phosphate. Approximately 98% of non-viral protein was removed …
The effect of non-aqueous solvents on the quaternary structure of viruses: properties of haemagglutinins obtained by disruption of influenza viruses with tri (n-butyl) …
AR Neurath - Microbios, 1970 - cabdirect.org
Influenza virus suspensions containing 40 000 HA units/ml. were disrupted by addition of 1:
1000 Tween 80 and by shaking with an equal volume of tri (n-butyl) phosphate, with the …
1000 Tween 80 and by shaking with an equal volume of tri (n-butyl) phosphate, with the …
Purification and aggregation of influenza virus by precipitation with polyethylene glycol
A Poison - Preparative Biochemistry, 1974 - Taylor & Francis
Influenza virus may be purified and rendered free of extraneous proteins by precipitation
and aggregation with polyethylene glycol at polymer concentrations of 1 to 4%. The …
and aggregation with polyethylene glycol at polymer concentrations of 1 to 4%. The …
A chromatographic procedure for the purification of influenza virus
DS Pepper - Journal of General Virology, 1967 - microbiologyresearch.org
Owing to the difficulties of achieving consistently good recoveries of virus from allantoic fluid
containing influenza virus by chromatography on calcium phosphate columns the factors …
containing influenza virus by chromatography on calcium phosphate columns the factors …
The rapid concentration and purification of influenza virus from allantoic fluid
JT Heyward, RA Klimas, MD Stapp, JF Obijeski - Archives of virology, 1977 - Springer
Influenza virus was quantitatively recovered from infectious allantoic fluid by precipitation
with 8 per cent (wt/vol) polyethylene glycol 6000 (PEG-6000). Virus concentrated by PEG …
with 8 per cent (wt/vol) polyethylene glycol 6000 (PEG-6000). Virus concentrated by PEG …
[PDF][PDF] Fixation of “neutralized” influenza virus by susceptible cells
JV Hultin, AP McKEE - Journal of Bacteriology, 1952 - Am Soc Microbiol
MATERIALS AND METHODS Active influenza virus. The type A str of virus used was
isolated in Iowa City during the 1943 epidemic. Fresh viral suspensions were obtained by …
isolated in Iowa City during the 1943 epidemic. Fresh viral suspensions were obtained by …
The structure of influenza viruses: 3. Disruption of the virus particle and separation of neuraminidase activity
WG Laver - Virology, 1963 - Elsevier
This paper describes methods for the disruption of particles of influenza B virus (LEE strain),
efficiently and without cleavage of covalent bonds, into subunits very much smaller than the …
efficiently and without cleavage of covalent bonds, into subunits very much smaller than the …
Use of zwitterionic detergent for the preparation of an influenza virus vaccine. 1: Preparation and characterization of disrupted virions
CR Crawford, FA Mukhlis, R Jenning, JS Oxford… - Vaccine, 1984 - Elsevier
Abstract The zwitterionic, Empigen BB® an alkylbetaine based on a C 12–C 14 alcohol was
shown to disrupt influenza A and B viruses in such a way as to retain the biological activity of …
shown to disrupt influenza A and B viruses in such a way as to retain the biological activity of …
Soluble antigens obtained from influenza virus by treatment with non-ionic detergent
MJ Corbel, CJM Rondle - Epidemiology & Infection, 1970 - cambridge.org
Highly purified influenza virus was degraded using anionic and non-ionic detergents. Best
results were obtained using the non-ionic detergent Triton N101. Tests showed that virus …
results were obtained using the non-ionic detergent Triton N101. Tests showed that virus …
Purification of influenza viruses on wide-pore glass columns.
SE Bresler, GI Bespalova, VM Kolikov, SP Zhdanov… - Acta …, 1975 - europepmc.org
Purification of influenza viruses on wide-pore glass columns. - Abstract - Europe PMC Sign in
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