Genetic circuits and metabolic pathways can be reengineered to allow organisms to process
signals and manufacture useful chemicals. However, their functions currently rely on …

Robust and predictably performing synthetic circuits rely on the use of well-characterized
regulatory parts across different genetic backgrounds and environmental contexts. Here we …

Highlights•Recently developed genome engineering tools facilitate pathway control.•Novel
genetic regulators enable multi-level static and dynamic control.•State-of-the-art applications …

The engineering of biological systems to achieve specific purposes requires design tools
that function in a predictable and quantitative manner. Recent advances in the field of …

Gene expression occurs in two essential steps: transcription and translation. In bacteria, the
two processes are tightly coupled in time and space, and highly regulated. Tight regulation …

Engineering of synthetic gene circuits is a rapidly growing discipline, currently dominated by
prokaryotic transcription networks, which can be easily rearranged or rewired to give …

Highlights•Synthetic biology approaches enable microbial systems engineering.•Versatile
tools rapidly expand regulatory engineering capabilities.•Modular components and …

Despite substantial progress in synthetic biology, we still lack the ability to engineer anything
as complex as Nature has. One of the many reasons is that we lack the requisite tools for …

A major goal of biological research is to provide a mechanistic understanding of diverse
biological processes. To this end, synthetic biology offers a powerful approach, whereby …

Synthetic biology approaches commonly introduce heterologous gene networks into a host
to predictably program cells, with the expectation of the synthetic network being orthogonal …