Publisher Summary This chapter describes the in vitro ADP-ribosylation reaction, the
purification of the C3 exoenzyme, and the application of the C3 exoenzyme in biological …

Clostridium botulinum C2 toxin is a member of the family of binary actin-ADP-ribosylating
toxins. It consists of the enzyme component C2I, and the separated binding/translocation …

RhoA,-B, and-C are ADP-ribosylated by Clostridium botulinum exoenzyme C3 to induce
redistribution of the actin filaments in intact cells, a finding that has led to the notion that the …

Mono ADP-ribosylation is a common characteristic of bacterial toxins resulting to aberrant
activation or inactivation of target proteins. The C3 exoenzyme of Clostridium botulinum …

Specific [32P] ADP-ribosylation by Clostridium botulinum exoenzyme C3 was used to study
the involvement of phosphorylation in the regulation of the low-molecular-mass GTP-binding …

Clostridium botulinum C2 toxin is the prototype of actin-ADP-ribosylating toxins. The toxin
consists of the enzyme component C2I and the separated binding/translocation component …

By cation-exchange column chromatography followed by gel filtration or hydroxylapatite
column chromatography, ADP-ribosyltransferases (exoenzyme C3) were isolated from …

Clostridium botulinum exoenzyme C3 inactivates the small GTPase Rho by ADP-
ribosylation. We used a C3 fusion toxin (C2IN-C3) with high cell accessibility to study the …

The family of C3-like exoenzymes comprises seven bacterial ADP-ribosyltransferases of
different origin. The common hallmark of these exoenzymes is the selective N-ADP …

ADP-ribosylation of Rho (A, B, C) by the family of exoenzyme C3-like transferases induces
reorganization of the actin cytoskeleton based on inactivation of RhoA. No data are …