The catalytic polypeptide of human DNA polymerase δ was overexpressed in BSC-40 cells
(African green monkey kidney cell line) using the vaccinia virus/pTM1 system. The …

A 12-kDa and two 25-kDa polypeptides were isolated with highly purified calf thymus DNA
polymerase δ by conventional chromatography. A 16-mer peptide sequence was obtained …

The cDNA of human DNA polymerase δ was cloned. The cDNA had a length of 3.5 kb and
encoded a protein of 1107 amino acid residues with a calculated molecular mass of 124 …

Mammalian DNA polymerase δ was originally characterized as a tightly associated
heterodimer consisting of the catalytic subunit, p125, and the p50 subunit. Recently, two …

Mammalian DNA polymerase δ (pol δ), a key enzyme of chromosomal DNA replication,
consists of four subunits as follows: the catalytic subunit; p125, which is tightly associated …

DNA polymerase δ consists of at least four subunits: p125, p68, p50, and p12 [Liu et al., J.
Biol. Chem. 275 (2000) 18739–18744]. We have isolated genomic DNA clones covering the …

Publisher Summary This chapter discusses the purification of mammalian polymerases,
DNA polymerase δ. DNA polymerase δ is one of three DNA polymerases required for …

The catalytic subunit of human DNA polymerase δ has been overexpressed in insect cells
by a recombinant baculovirus. The recombinant protein has a M r=~ 125,000 and is …

The BRCA1 COOH terminus (BRCT) motif is present in many nuclear proteins that contribute
to cell cycle regulation or DNA repair. Polymerase chain reaction-based screening with …

We have isolated genomic DNA clones covering the gene for human DNA polymerase δ
catalytic subunit (POLD1) and its 5′ flanking sequence. This gene is divided into 27 exons …