Rotaviruses are enteric pathogens responsible for a significant burden of disease,
especially in children, through person-to-person transmission and exposure to contaminated …

Six‐hundred and twenty‐six stool specimens collected from children with diarrhea over a 12‐
month period were tested for rotavirus using a real time reverse transcription‐polymerase …

A sensitive and specific real‐time RT‐PCR assay to detect rotavirus in stool samples was
optimized and validated using a wide range of rotavirus genotypes. The target of the original …

Rotaviruses are one of the major causes of diarrhea in infants and children under 5 years
old, especially affecting developing countries. In natural disasters, fecal matter and potable …

Seven commercial rotavirus antigen assays were compared with in-house PCR methods for
detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the …

Water samples were concentrated by the modified adsorption–elution technique followed by
speedVac reconcentration of the filter eluates. Reverse transcriptase-nested polymerase …

Techniques such as the real-time reverse transcription-polymerase chain reaction (qRT-
PCR) can detect RNA in samples with a low viral load. However, these amplicons typically …

Our aim was to evaluate the reverse transcription and polymerase chain reaction (RT/PCR)
technique for the detection of rotavirus shedding by infected children as a routine diagnostic …

The standard diagnosis of rotavirus gastroenteritis is based on the demonstration of
rotavirus antigen in stools using an enzyme immunoassay (EIA). In this study, a one-step …

Nucleic acid amplification techniques are used frequently for rapid diagnosis of viral
diseases. In this study, a real-time polymerase chain reaction protocol that uses primers …