Here we report the generation and use of three multisite Gateway TA-cloning plasmids, p5E-
TA, pME-TA, and p3E-TA, to enable rapid and cheap construction of new entry vectors and …

Transgenesis is an important methodology for studying the function of genes and genomes
in model plants and animals. For the model vertebrate zebrafish, methods using the Tol2 …

DNA transposons are effective chromosomal engineering vehicles for making transgenic
zebrafish. We describe both autonomous and non-autonomous transposable elements, and …

Transgenesis, which inserts exogenous DNA into animal genomes, is a widely used
technique. Traditionally, the constructs for transgenesis are generated by step-by-step …

The transgenesis and gene and enhancer trap: Methods in zebrafish by using the Tol2
transposable element Page 172 CHAPTER 8 The Transgenesis and Gene and Enhancer Trap …

Transgenesis of large DNA constructs is essential for gene function analysis. Recently, Tol2
transposase-mediated transgenesis has emerged as a powerful tool to insert bacterial …

Transgenesis is an important tool for assessing gene function. In zebrafish, transgenesis has
suffered from three problems: the labor of building complex expression constructs using …

The zebrafish (Danio rerio) is a useful model for genetic studies of vertebrate development.
Its embryos are transparent and develop rapidly outside the mother, making it feasible to …

Abstract Background The Tol2 transposable element is the most widely used transgenesis
tool in zebrafish. However, its high activity almost always leads to multiple unlinked …

The generation of zebrafish transgenic lines that express specific fluorophores in a cell-or
tissue-specific manner is an important technique that takes full advantage of the optical …