State-of-the-art CRISPR for in vivo and cell-based studies in Drosophila
J Zirin, J Bosch, R Viswanatha, SE Mohr, N Perrimon - Trends in Genetics, 2022 - cell.com
For more than 100 years, the fruit fly, Drosophila melanogaster, has served as a powerful
model organism for biological and biomedical research due to its many genetic and …
model organism for biological and biomedical research due to its many genetic and …
Systematic Evaluation of Drosophila CRISPR Tools Reveals Safe and Robust Alternatives to Autonomous Gene Drives in Basic Research
F Port, N Muschalik, SL Bullock - G3: Genes, Genomes …, 2015 - academic.oup.com
Abstract The Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR
associated (CRISPR/Cas) technology allows rapid, site-specific genome modification in a …
associated (CRISPR/Cas) technology allows rapid, site-specific genome modification in a …
Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila
SJ Gratz, FP Ukken, CD Rubinstein, G Thiede… - Genetics, 2014 - academic.oup.com
We and others recently demonstrated that the readily programmable CRISPR/Cas9 system
can be used to edit the Drosophila genome. However, most applications to date have relied …
can be used to edit the Drosophila genome. However, most applications to date have relied …
[HTML][HTML] A genome-wide CRISPR library for high-throughput genetic screening in Drosophila cells
AR Bassett, L Kong, JL Liu - Journal of Genetics and Genomics, 2015 - Elsevier
The simplicity of the CRISPR/Cas9 system of genome engineering has opened up the
possibility of performing genome-wide targeted mutagenesis in cell lines, enabling …
possibility of performing genome-wide targeted mutagenesis in cell lines, enabling …
Creating Heritable Mutations in Drosophila with CRISPR-Cas9
F Port, SL Bullock - Drosophila: Methods and Protocols, 2016 - Springer
Reverse genetics—the creation of mutations in preselected target genes—has until recently
been a bottleneck in many Drosophila projects. The advent of clustered, regularly …
been a bottleneck in many Drosophila projects. The advent of clustered, regularly …
A toolkit of CRISPR-based genome editing systems in Drosophila
The last couple of years have witnessed an explosion in development of CRISPR-based
genome editing technologies in cell lines as well as in model organisms. In this review, we …
genome editing technologies in cell lines as well as in model organisms. In this review, we …
A mouse geneticist's practical guide to CRISPR applications
CRISPR/Cas9 system of RNA-guided genome editing is revolutionizing genetics research in
a wide spectrum of organisms. Even for the laboratory mouse, a model that has thrived …
a wide spectrum of organisms. Even for the laboratory mouse, a model that has thrived …
On-target CRISPR/Cas9 activity can cause undesigned large deletion in mouse zygotes
A Korablev, V Lukyanchikova, I Serova… - International Journal of …, 2020 - mdpi.com
Genome engineering has been tremendously affected by the appearance of the clustered
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 …
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 …
Robust CRISPR/Cas9-Mediated Tissue-Specific Mutagenesis Reveals Gene Redundancy and Perdurance in Drosophila
The CRISPR/Cas9 gene editing system continues to push the boundaries of genetic
analysis. Here, papers from Farboud, Severson, and Meyer and Poe et al. describe cutting …
analysis. Here, papers from Farboud, Severson, and Meyer and Poe et al. describe cutting …
CRISPR technology: A decade of genome editing is only the beginning
JY Wang, JA Doudna - Science, 2023 - science.org
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome
editing, coupled with advances in computing and imaging capabilities, has initiated a new …
editing, coupled with advances in computing and imaging capabilities, has initiated a new …