Cryopreservation of Mesenchymal Stem/Stromal Cells using a DMSO-free Solution is Comparable to DMSO-containing Cryoprotectants: Results of An International …

T Mamo, CA Cox, C Demorest, MJ Fontaine, A Hubel… - Cytotherapy, 2024 - Elsevier
Abstract Background and Aim An essential aspect of ensuring availability and stability of
mesenchymal stem/stromal cells (MSCs) products for clinical use is that these cells are …

Cryopreservation and revival of human mesenchymal stromal cells

M Haack-Sørensen, A Ekblond, J Kastrup - Mesenchymal Stem Cells …, 2016 - Springer
Cell-based therapy is a promising and innovative new treatment for different degenerative
and autoimmune diseases, and mesenchymal stromal cells (MSCs) from the bone marrow …

Cryopreservation of mesenchymal stem cells (MSCs) derived from bone marrow with carbohydrate additive sucrose and dimethyl sulfoxide (DMSO)

S Tripathy, S Singh, SK Das - Contemporary Medical Biotechnology …, 2022 - Elsevier
Effective cryopreservation strategies for mesenchymal stem cells (MSCs) are highly
essential to be used in future “off-the-shelf” therapeutic purposes. The bone marrow-derived …

Cryopreservation and revival of mesenchymal stromal cells

M Haack-Sørensen, J Kastrup - Mesenchymal Stem Cell Assays and …, 2011 - Springer
Over the past few years, the pace of preclinical stem cell research is astonishing and adult
stem cells have become the subject of intense research. Due to the presence of promising …

Dimethyl sulfoxide-free cryopreservation solutions for hematopoietic stem cell grafts

R Kaushal, S Jahan, C McGregor, N Pineault - Cytotherapy, 2022 - Elsevier
Background aims The use of effective methods for the cryopreservation of hematopoietic
stem cells (HSCs) is vital to retain the maximum engraftment activity of cord blood units …

Cryopreservation of human bone marrow‐derived mesenchymal stem cells with reduced dimethylsulfoxide and well‐defined freezing solutions

Y Liu, X Xu, X Ma, E Martin‐Rendon… - Biotechnology …, 2010 - Wiley Online Library
The aim of this study is to investigate the feasibility of using well defined, serum‐free
freezing solutions with a reduced level of dimethylsulfoxide (DMSO) of 7.5, 5, and 2.5%(v/v) …

[HTML][HTML] Reconstitution and post-thaw storage of cryopreserved human mesenchymal stromal cells: Pitfalls and optimizations for clinically compatible formulants

RR Aabling, T Alstrup, EM Kjær, KJ Poulsen… - Regenerative …, 2023 - Elsevier
Introduction The regenerative and immunomodulatory properties of multipotent
mesenchymal stromal cells (MSCs) make them an intriguing asset for therapeutic …

Novel Cryopreservation Approach Providing Off‐the‐Shelf Availability of Human Multipotent Mesenchymal Stromal Cells for Clinical Applications

O Rogulska, O Tykhvynska, O Revenko… - Stem Cells …, 2019 - Wiley Online Library
Cryopreservation is the only established method to provide long‐term storage and fast
availability of cellular product for therapeutic applications. The overwhelming majority of …

Defined serum-and xeno-free cryopreservation of mesenchymal stem cells

SH Al-Saqi, M Saliem, HC Quezada, Å Ekblad… - Cell and tissue …, 2015 - Springer
Mesenchymal stem cells (MSCs) have vast potential in cell therapy, and are experimentally
used in the clinic. Therefore, it is critical to find a serum-and xeno-free cryopreservation …

Mesenchymal stromal cell cryopreservation

S Renzi, T Lombardo, S Dotti, SS Dessì… - Biopreservation and …, 2012 - liebertpub.com
The advent of stem cells and stem cell-based therapies for specific diseases requires
particular knowledge of laboratory procedures, which not only guarantee the continuous …