The versatility of clustered regularly interspaced short palindromic repeat (CRISPR)-
associated (Cas) genome editing makes it a popular tool for many research and …

The recent discovery and use of CRISPR/Cas9 gene editing technology has provided new
opportunities for scientific research in many fields of study including agriculture, genetic …

Clustered regularly interspaced short palindromic repeats (CRISPR)–Cas9 technology is an
important tool for genome editing because the Cas9 endonuclease can induce targeted …

Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome
engineering, while also changing the speed and efficiency of metabolic engineering in …

CRISPR/Cas9 technology allows accurate, marker-less genome editing. We report a
detailed, robust, and streamlined protocol for CRISPR/Cas9 genome editing in …

Recent advances in CRISPR/Cas9 based genome editing have considerably advanced
genetic engineering of industrial yeast strains. In this study, we report the construction and …

ABSTRACT CRISPR-Cas9 genome editing technology is widely used in scientific research
and biotechnology. As this technology becomes a staple tool in life sciences research, it is …

CRISPR‐Cas9 has emerged as a powerful method for editing the genome in a wide variety
of species, since it can generate a specific DNA break when targeted by the Cas9‐bound …

CRISPR-Cas has become a powerful technique for genetic engineering of yeast. Here, we
present an improved version by using only one single plasmid expressing Cas9 and one or …

The methods currently employed for in vivo site-directed mutagenesis in yeast are laborious
and/or inefficient. Recent developments of the CRISPR-based approaches hold great …