Two atypical mobilization proteins are involved in plasmid CloDF13 relaxation
B Núñez, F De La Cruz - Molecular microbiology, 2001 - Wiley Online Library
The mobilization region of plasmid CloDF13 was localized to a 3.6 kb DNA segment that
was analysed by transposon mutagenesis and DNA sequencing. Analysis of the DNA …
was analysed by transposon mutagenesis and DNA sequencing. Analysis of the DNA …
Investigating the basis of substrate recognition in the pC221 relaxosome
JA Caryl, CD Thomas - Molecular microbiology, 2006 - Wiley Online Library
The nicking of the origin of transfer (oriT) is an essential initial step in the conjugative
mobilization of plasmid DNA. In the case of staphylococcal plasmid pC221, nicking by the …
mobilization of plasmid DNA. In the case of staphylococcal plasmid pC221, nicking by the …
The relaxosome protein MobC promotes conjugal plasmid mobilization by extending DNA strand separation to the nick site at the origin of transfer
S Zhang, R Meyer - Molecular microbiology, 1997 - Wiley Online Library
The frequency of conjugal mobilization of plasmid R1162 is decreased approximately 50‐
fold if donor cells lack MobC, one of the plasmid‐encoded proteins making up the …
fold if donor cells lack MobC, one of the plasmid‐encoded proteins making up the …
MobB protein stimulates nicking at the R1162 origin of transfer by increasing the proportion of complexed plasmid DNA
T Perwez, R Meyer - Journal of bacteriology, 1996 - Am Soc Microbiol
An essential early step in conjugal mobilization of R1162, nicking of the DNA strand that is
subsequently transferred, is carried out in the relaxosome, a complex of two plasmid …
subsequently transferred, is carried out in the relaxosome, a complex of two plasmid …
Stabilization of the relaxosome and stimulation of conjugal transfer are genetically distinct functions of the R1162 protein MobB
T Perwez, RJ Meyer - Journal of bacteriology, 1999 - Am Soc Microbiol
MobB is a small protein encoded by the broad-host-range plasmid R1162 and required for
efficient mobilization of its DNA during conjugation. The protein was shown previously to …
efficient mobilization of its DNA during conjugation. The protein was shown previously to …
Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking accessory proteins
A Varsaki, G Moncalián… - Journal of …, 2009 - Am Soc Microbiol
MbeC is a 13-kDa ColE1-encoded protein required for efficient mobilization of ColE1, a
plasmid widely used in cloning vector technology. MbeC protein was purified and used for in …
plasmid widely used in cloning vector technology. MbeC protein was purified and used for in …
Stringent and Relaxed Recognition of oriT by Related Systems for Plasmid Mobilization: Implications for Horizontal Gene Transfer
S Jandle, R Meyer - Journal of bacteriology, 2006 - Am Soc Microbiol
The plasmids R1162 and pSC101 have origins of conjugative transfer (oriT s) and
corresponding relaxases that are closely related. The oriT s are made up of a highly …
corresponding relaxases that are closely related. The oriT s are made up of a highly …
Localized denaturation of oriT DNA within relaxosomes of the broad‐host‐range plasmid R1162
S Zhang, RJ Meyer - Molecular microbiology, 1995 - Wiley Online Library
The broad‐host‐range, multicopy plasmid R1162 is efficiently mobilized during conjugation
by the self‐transmissible plasmid R751. The relaxosome, a complex of plasmid DNA and …
by the self‐transmissible plasmid R751. The relaxosome, a complex of plasmid DNA and …
Purification of the large mobilization protein of plasmid RSF1010 and characterization of its site‐specific DNA‐cleaving/DNA‐joining activity
E Scherzinger, V Kruft, S Otto - European journal of …, 1993 - Wiley Online Library
A site‐specific and strand‐specific nick, introduced into the RSF1010 plasmid origin of
transfer (oriT), initiates unidirectional DNA transfer during bacterial conjugation. We have …
transfer (oriT), initiates unidirectional DNA transfer during bacterial conjugation. We have …
Genes involved in conjugative DNA processing of plasmid R6K
B Núñez, P Avila, F De La Cruz - Molecular microbiology, 1997 - Wiley Online Library
The conjugative transfer region of the IncX plasmid R6K (TRAX) was analysed by
transposon mutagenesis and DNA sequencing. Tn5tac1 insertional mutations localized …
transposon mutagenesis and DNA sequencing. Tn5tac1 insertional mutations localized …