Culture conditions affect meiotic regulation in cumulus cell‐enclosed mouse oocytes

SM Downs, AM Mastropolo - Molecular Reproduction and …, 1997 - Wiley Online Library
To test the hypothesis that culture conditions influence meiotic regulation in mouse oocytes,
we have examined the effects of six culture media, four organic buffers, and pH on …

Requirement for glucose in ligand-stimulated meiotic maturation of cumulus cell-enclosed mouse oocytes

CF Fagbohun, SM Downs - Reproduction, 1992 - rep.bioscientifica.com
In this study, the effect of different energy sources used in Eagle's minimum essential
medium on the meiotic maturation of mouse oocytes in culture was examined. The effects of …

Involvement of purine nucleotide synthetic pathways in gonadotropin‐induced meiotic maturation in mouse cumulus cell‐enclosed oocytes

SM Downs - Molecular Reproduction and Development …, 1997 - Wiley Online Library
This study was carried out to test the hypothesis that purine nucleotide‐generating pathways
are required for ligand‐stimulated oocyte maturation in meiotically arrested cumulus cell …

Mouse versus rat: profound differences in meiotic regulation at the level of the isolated oocyte

SM Downs - Molecular reproduction and development, 2011 - Wiley Online Library
Cumulus cell‐enclosed oocytes (CEO), denuded oocytes (DO), or dissected follicles were
obtained 44–48 hr after priming immature mice (20–23 days old) with 5 IU or immature rats …

Meiotic resumption and gap junction modulation in the cultured rat cumulus‐oocyte complex

SE Wert, WJ Larsen - Gamete research, 1989 - Wiley Online Library
The mammalian oocyte is normally arrested in the dictyate stage of the first meiotic division
and resumes meiosis in vivo in response to the appropriate hormonal signals [Ayalon et al …

Developmental capacity of mouse oocytes following maintenance of meiotic arrest in vitro

SM Downs, AC Schroeder, JJ Eppig - Gamete research, 1986 - Wiley Online Library
It was shown previously that the frequencies of fertilization and pre‐and post‐implantation
embryonic development of mouse oocytes matured in vitro were similar to those of oocytes …

Induction of maturation in cumulus cell‐enclosed mouse oocytes by follicle‐stimulating hormone and epidermal growth factor: Evidence for a positive stimulus of …

SM Downs, SAJ Daniel, JJ Eppig - Journal of Experimental …, 1988 - Wiley Online Library
The efficacy of follicle‐stimulating hormone (FSH), epidermal growth factor (EGF), and
dibutyryl cGMP (dbcGMP) as inducers of germinal vesicle breakdown (GVBD) in cumulus …

Glutamine and the maintenance of meiotic arrest in mouse oocytes: influence of culture medium, glucose, and cumulus cells

SM Downs, A Verhoeven - Molecular Reproduction and …, 2003 - Wiley Online Library
The selection of culture media and supplements therein has a tremendous impact on the
regulation of oocyte maturation in vitro. In the present study, we have evaluated how altering …

Acquisition of meiotic competence by denuded mouse oocytes: participation of somatic-cell product (s) and cAMP

F Chesnel, K Wigglesworth, JJ Eppig - Developmental biology, 1994 - Elsevier
In contrast to fully grown oocytes, growing mouse oocytes are not capable of undergoing
germinal vesicle breakdown (GVB) when released from the follicle unless they are first …

Cumulus cells of oocyte‐cumulus complexes secrete a meiosis‐activating substance when stimulated with FSH

AG Byskov, CY Andersen, A Hossaini… - Molecular …, 1997 - Wiley Online Library
The effect of the different follicular cell types on resumption of meiosis was studied during
stimulation with FSH. Cumulus enclosed oocytes (CEO), denuded oocytes (DO), and …