Construction of transposon Tn3phoA: its application in defining the membrane topology of the Agrobacterium tumefaciens DNA transfer proteins
A Das, YH Xie - Molecular microbiology, 1998 - Wiley Online Library
Protein fusion with the Escherichia coli alkaline phosphatase is used extensively for the
analysis of the topology of membrane proteins. To study the topology of the Agrobacterium T …
analysis of the topology of membrane proteins. To study the topology of the Agrobacterium T …
Construction and characterization of Tn5virB, a transposon that generates nonpolar mutations, and its use to define virB8 as an essential virulence gene in …
EM Dale, AN Binns, JE Ward Jr - Journal of bacteriology, 1993 - Am Soc Microbiol
Transfer of the T-DNA from the Ti plasmid of Agrobacterium tumefaciens into plant cells
involves movement of a single-stranded DNA-protein intermediate across several …
involves movement of a single-stranded DNA-protein intermediate across several …
Subcellular localization of the Agrobacterium tumefaciens T‐DNA transport pore proteins: VirB8 is essential for the assembly of the transport pore
RB Kumar, YH Xie, A Das - Molecular microbiology, 2000 - Wiley Online Library
Agrobacterium tumefaciens transforms plants by transferring DNA to the plant cell nucleus.
The VirB membrane proteins are postulated to form a pore for the transport of the DNA …
The VirB membrane proteins are postulated to form a pore for the transport of the DNA …
Subcellular localization of seven VirB proteins of Agrobacterium tumefaciens: implications for the formation of a T-DNA transport structure
YR Thorstenson, GA Kuldau… - Journal of …, 1993 - Am Soc Microbiol
Plant cell transformation by Agrobacterium tumefaciens involves the transfer of a single-
stranded DNA-protein complex (T-complex) from the bacterium to the plant cell. One of the …
stranded DNA-protein complex (T-complex) from the bacterium to the plant cell. One of the …
Functional Analysis of the Agrobacterium tumefaciens T-DNA Transport Pore Protein VirB8
RB Kumar, A Das - Journal of bacteriology, 2001 - Am Soc Microbiol
ABSTRACT The VirB8 protein of Agrobacterium tumefaciens is essential for DNA transfer to
plants. VirB8, a 237-residue polypeptide, is an integral membrane protein with a short N …
plants. VirB8, a 237-residue polypeptide, is an integral membrane protein with a short N …
The Agrobacterium T-DNA Transport Pore Proteins VirB8, VirB9, and VirB10 Interact with One Another
A Das, YH Xie - Journal of bacteriology, 2000 - Am Soc Microbiol
ABSTRACT The VirB proteins of Agrobacterium tumefaciens form a transport pore to transfer
DNA from bacteria to plants. The assembly of the transport pore will require interaction …
DNA from bacteria to plants. The assembly of the transport pore will require interaction …
Polar location and functional domains of the Agrobacterium tumefaciens DNA transfer protein VirD4
RB Kumar, A Das - Molecular microbiology, 2002 - Wiley Online Library
Agrobacterium tumefaciens VirD4 is essential for DNA transfer to plants. VirD4 presumably
functions as a coupling factor that facilitates communication between a substrate and the …
functions as a coupling factor that facilitates communication between a substrate and the …
Dimerization of the Agrobacterium tumefaciens VirB4 ATPase and the effect of ATP‐binding cassette mutations on the assembly and function of the T‐DNA …
TA Dang, XR Zhou, B Graf… - Molecular microbiology, 1999 - Wiley Online Library
The Agrobacterium tumefaciens VirB4 ATPase functions with other VirB proteins to export T‐
DNA to susceptible plant cells and other DNA substrates to a variety of prokaryotic and …
DNA to susceptible plant cells and other DNA substrates to a variety of prokaryotic and …
Delineation of the interaction domains of Agrobacterium tumefaciens VirB7 and VirB9 by use of the yeast two-hybrid assay
A Das, LB Anderson, YH Xie - Journal of bacteriology, 1997 - Am Soc Microbiol
The Agrobacterium tumefaciens VirB proteins are postulated to form a transport pore for the
transfer of T-DNA. Formation of the transport pore will involve interactions among the VirB …
transfer of T-DNA. Formation of the transport pore will involve interactions among the VirB …
The VirB4 ATPase of Agrobacterium tumefaciens is a cytoplasmic membrane protein exposed at the periplasmic surface
TA Dang, PJ Christie - Journal of bacteriology, 1997 - Am Soc Microbiol
The VirB4 ATPase of Agrobacterium tumefaciens, a putative component of the T-complex
transport apparatus, associates with the cytoplasmic membrane independently of other …
transport apparatus, associates with the cytoplasmic membrane independently of other …